Figure 2

2D-DIGE-based protein expression analysis. Pseudo-colored images were generated using the Multi-Channel Viewer function of the PDQuest software suite. Four sample sets that were either unstimulated or stimulated with the Mtb-specific antigen or mitogen from patients with active pulmonary TB and four corresponding sets from control subjects were used to screen for candidate proteins by 2D-DIGE. A: Analysis based on differences in levels in samples from patients and control subjects. Red indicates proteins whose levels increased and green indicates proteins whose levels decreased in unstimulated supernatants from patients with active TB compared with control subjects. Spot (a): HT6102, Spot (b): HT2406, and Spot (c): HT5401 correspond to the PDQuest identification numbers shown in Table 2. B: Analysis based on differences in levels after stimulation. Red indicates proteins whose levels increased in whole blood supernatants from patients with active TB after incubation with Mtb-specific antigens. Spots (d): T3107, T4203, and T4208, correspond to Table 2.