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Table 2 The performance of b-EDIII-T as a capture antigen in the detection of anti-DENV antibodiesa

From: Evaluation of envelope domain III-based single chimeric tetravalent antigen and monovalent antigen mixtures for the detection of anti-dengue antibodies in human sera

  IgG (n = 80) IgM (n = 81)
Parameter PS SA Mean Ratio of S/Co
values (95% CI)b
PS SA Mean Ratio of S/Co
values (95% CI)b
% Sensitivityc 82.5 91.3 1.32 (1.24-1.39) 61.7 75.3 1.30 (1.23-1.37)
% Specificityd 100 100 - 100 100 -
  1. aAssays were done using polystyrene (PS) microtiter wells or streptavidin (SA) coated microtiter wells. The number of sera analyzed is denoted by n.
  2. bThis column represents the mean ratio of S/Co values using SA plates to S/Co values using PS plates; the cut-off values were 0.31 and 0.28 for PS and SA, respectively, in the IgG assay, and 0.23 and 0.25, respectively, in the IgM assay. The 95% confidence interval is indicated in parentheses.
  3. cPercent Sensitivity = (Number of sera that scored positive by our test/Number of sera that scored positive using PanBio ELISAs) × 100
  4. dPercent Specificity = (Number of sera that scored negative by our test/Number of sera that scored negative using PanBio ELISAs) × 100
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