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Table 1 Comparison of EDIII-based monovalent antigen mixture and single tetravalent antigen in anti-DENV antibody detectiona

From: Evaluation of envelope domain III-based single chimeric tetravalent antigen and monovalent antigen mixtures for the detection of anti-dengue antibodies in human sera

  IgG (n = 80) IgM (n = 81)
Parameter EDIII Mix EDIII-T Mean Ratio of S/Co
values (95% CI)b
EDIII Mix EDIII-T Mean Ratio of S/Co
values (95% CI)b
% Sensitivityc 81.3 82.5 1.22 (1.15-1.29) 59.3 63.0 1.11 (1.06-1.16)
% Specificityd 100 100 - 100 100 -
  1. aAssays were done using polystyrene microtiter wells that were coated either with a mixture of four EDIII monovalent antigens (EDIII Mix) or the single tetravalent antigen, EDIII-T. The number of sera analyzed is denoted by n.
  2. bThis column represents the mean ratio of S/Co values using EDIII-T to S/Co values using EDIII Mix as the coating antigens; the cut-off values were 0.30 and 0.33 for EDIII mix and EDIII-T, respectively, in the IgG assay, and 0.23 and 0.25, respectively, in the IgM assay. The 95% confidence interval is indicated in parentheses.
  3. cPercent Sensitivity = (Number of sera that scored positive by our test/Number of sera that scored positive using PanBio ELISAs) × 100
  4. dPercent Specificity = (Number of sera that scored negative by our test/Number of sera that scored negative using PanBio ELISAs) × 100
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