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Table 1 Comparison of EDIII-based monovalent antigen mixture and single tetravalent antigen in anti-DENV antibody detectiona

From: Evaluation of envelope domain III-based single chimeric tetravalent antigen and monovalent antigen mixtures for the detection of anti-dengue antibodies in human sera

 

IgG (n = 80)

IgM (n = 81)

Parameter

EDIII Mix

EDIII-T

Mean Ratio of S/Co

values (95% CI)b

EDIII Mix

EDIII-T

Mean Ratio of S/Co

values (95% CI)b

% Sensitivityc

81.3

82.5

1.22 (1.15-1.29)

59.3

63.0

1.11 (1.06-1.16)

% Specificityd

100

100

-

100

100

-

  1. aAssays were done using polystyrene microtiter wells that were coated either with a mixture of four EDIII monovalent antigens (EDIII Mix) or the single tetravalent antigen, EDIII-T. The number of sera analyzed is denoted by n.
  2. bThis column represents the mean ratio of S/Co values using EDIII-T to S/Co values using EDIII Mix as the coating antigens; the cut-off values were 0.30 and 0.33 for EDIII mix and EDIII-T, respectively, in the IgG assay, and 0.23 and 0.25, respectively, in the IgM assay. The 95% confidence interval is indicated in parentheses.
  3. cPercent Sensitivity = (Number of sera that scored positive by our test/Number of sera that scored positive using PanBio ELISAs) × 100
  4. dPercent Specificity = (Number of sera that scored negative by our test/Number of sera that scored negative using PanBio ELISAs) × 100