Figure 2
Stability of influenza RNA at room temperature and 4 C when spiked into buffy coats at different concentrations. 3.55 × 106 (A), 3.55 × 105 (B), or 3.55 × 104 (C) EID50 of H1N1 virus in 10 μl of PBS, diluted as stated in Materials and Methods was mixed with 130 μl of buffy-coated buffy coats for different periods of time indicated. Real-time RT-PCR was performed with TaqMan, and the results were shown as Ct value in RT-PCR.