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Table 2 Multi-centre evaluation of Speed-oligo Mycobacteria and comparison to Genotype CM

From: Multi-centre evaluation of the speed-oligo Mycobacteria assay for differentiation of Mycobacteriumspp. in clinical isolates

Mycobacterium species a no. of Strains   Speed-oligo results Genotype CM results d
   Bands b Identification c  
IML Gauting, Germany 59    
   M. tuberculosis e 13 (3), 4, 7 M. tuberculosis complex M. tuberculosis complex
   M. avium 14 5, 7 MAIS group M. avium
   M. intracellulare 9 5, 7 MAIS group M. intracellulare
   M. intracellulare 1 5, 7 MAIS group Mycobacterium spp.
   M. kansasii 7 3, 7 M. kansasii M. kansasii
   M. kansasii 1 3, 7 M. kansasii Mycobacterium spp.
   M. abscessus 1 1, 7 M. chelonae/M. abscessus complex M. abscessus
   M. chelonae 1 1, 7 M. chelonae/M. abscessus complex M. chelonae
   M. gordonae 7 2, 7 M. gordonae M. gordonae
   M. fortuitum 2 6, 7 M. fortuitum M. fortuitum
   M. peregrinum 1 (6), 7 M. fortuitum M. peregrinum
   M. xenopi 1 7 Mycobacterium spp. M. xenopi
   Tsukamurella pulmonis 1 - no Mycobacterium spp. Gram+ bacteria, high G+C content
Bioscientia Ingelheim, Germany 60    
   M. tuberculosis e 13 (3), 4, 7 M. tuberculosis complex M. tuberculosis complex
   M. bovis BCGe 1 (3), 4, 7 M. tuberculosis complex M. tuberculosis complex
   M. avium 4 5, 7 MAIS group M. avium
   M. intracellulare 6 5, 7 MAIS group M. intracellulare
   M. scrofulaceum 1 5, 7 MAIS group M. scrofulaceum
   M. kansasii 1 3, 7 M. kansasii M. kansasii
   M. chelonae 3 1, 7 M. chelonae/M. abscessus complex M. chelonae
   M. gordonae 14 2, 7 M. gordonae M. gordonae
   M. fortuitum 2 6, 7 M. fortuitum M. fortuitum
   M. celatum 4 7 Mycobacterium spp. Mycobacterium spp.
   M. flavescens 1 7 Mycobacterium spp. Mycobacterium spp.
   M. malmoense 1 7 Mycobacterium spp M. malmoense
   M. montefiorense 1 7 Mycobacterium spp. Mycobacterium spp.
   M. peregrinum 1 6, 7 M. fortuitum M. peregrinum
   M. simiae 3 7 Mycobacterium spp. Mycobacterium spp.
   M. xenopi 2 7 Mycobacterium spp. M. xenopi
   Nocardia spp. 2 - no Mycobacterium spp. n.d.
KLINLAB Prague, Czech Rep. 75    
   M. tuberculosis e 26 (3), 4, 7 M. tuberculosis complex M. tuberculosis complex
   M. bovis BCGe 1 (3), 4, 7 M. tuberculosis complex M. tuberculosis complex
   M. avium 13 5, 7 MAIS group M. avium
   M. intracellulare 5 5, 7 MAIS group M. intracellulare
   M. kansasii 3 3, 7 M. kansasii M. kansasii
   M. abscessus 1 1, 7 M. chelonae/M. abscessus complex M. abscessus
   M. chelonae 1 1, 7 M. chelonae/M. abscessus complex M. chelonae
   M. gordonae 9 2, 7 M. gordonae M. gordonae
   M. fortuitum 9 6, 7 M. fortuitum M. fortuitum
   M. lentiflavum 2 7 Mycobacterium spp. Mycobacterium spp.
   M. marinum 1 3, 7 M. kansasii M. marinum
   M. xenopi 4 7 Mycobacterium spp. M. xenopi
Khartoum, Sudan 48    
   M. tuberculosis e 16 (3), 4, 7 M. tuberculosis complex M. tuberculosis complex
   M. tuberculosis e + M. fortuitum 7 (3), 4, 6, 7 M. tuberculosis complex + M. fortuitum M. tuberculosis complex + M. fortuitum
   M. fortuitum 25 6, 7 M. fortuitum M. fortuitum
TOTAL 242    
  1. aDifferentiation to species level by use of Genotype CM, Genotype AS, Genotype MTBC or sequencing (16S rDNA or hsp65 gene).
  2. bNumbers in brackets indicate weaker staining.
  3. cMAIS group: M. avium M. intracellulare M. scrofulaceum group.
  4. dIdentification results by Genotype CM are shown. n.d.: not determined.
  5. eAll MTBC isolates yielded weak TL3 signals in addition to TL4