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Table 2 Multi-centre evaluation of Speed-oligo Mycobacteria and comparison to Genotype CM

From: Multi-centre evaluation of the speed-oligo Mycobacteria assay for differentiation of Mycobacteriumspp. in clinical isolates

Mycobacterium species a

no. of Strains

 

Speed-oligo results

Genotype CM results d

  

Bands b

Identification c

 

IML Gauting, Germany

59

   

   M. tuberculosis e

13

(3), 4, 7

M. tuberculosis complex

M. tuberculosis complex

   M. avium

14

5, 7

MAIS group

M. avium

   M. intracellulare

9

5, 7

MAIS group

M. intracellulare

   M. intracellulare

1

5, 7

MAIS group

Mycobacterium spp.

   M. kansasii

7

3, 7

M. kansasii

M. kansasii

   M. kansasii

1

3, 7

M. kansasii

Mycobacterium spp.

   M. abscessus

1

1, 7

M. chelonae/M. abscessus complex

M. abscessus

   M. chelonae

1

1, 7

M. chelonae/M. abscessus complex

M. chelonae

   M. gordonae

7

2, 7

M. gordonae

M. gordonae

   M. fortuitum

2

6, 7

M. fortuitum

M. fortuitum

   M. peregrinum

1

(6), 7

M. fortuitum

M. peregrinum

   M. xenopi

1

7

Mycobacterium spp.

M. xenopi

   Tsukamurella pulmonis

1

-

no Mycobacterium spp.

Gram+ bacteria, high G+C content

Bioscientia Ingelheim, Germany

60

   

   M. tuberculosis e

13

(3), 4, 7

M. tuberculosis complex

M. tuberculosis complex

   M. bovis BCGe

1

(3), 4, 7

M. tuberculosis complex

M. tuberculosis complex

   M. avium

4

5, 7

MAIS group

M. avium

   M. intracellulare

6

5, 7

MAIS group

M. intracellulare

   M. scrofulaceum

1

5, 7

MAIS group

M. scrofulaceum

   M. kansasii

1

3, 7

M. kansasii

M. kansasii

   M. chelonae

3

1, 7

M. chelonae/M. abscessus complex

M. chelonae

   M. gordonae

14

2, 7

M. gordonae

M. gordonae

   M. fortuitum

2

6, 7

M. fortuitum

M. fortuitum

   M. celatum

4

7

Mycobacterium spp.

Mycobacterium spp.

   M. flavescens

1

7

Mycobacterium spp.

Mycobacterium spp.

   M. malmoense

1

7

Mycobacterium spp

M. malmoense

   M. montefiorense

1

7

Mycobacterium spp.

Mycobacterium spp.

   M. peregrinum

1

6, 7

M. fortuitum

M. peregrinum

   M. simiae

3

7

Mycobacterium spp.

Mycobacterium spp.

   M. xenopi

2

7

Mycobacterium spp.

M. xenopi

   Nocardia spp.

2

-

no Mycobacterium spp.

n.d.

KLINLAB Prague, Czech Rep.

75

   

   M. tuberculosis e

26

(3), 4, 7

M. tuberculosis complex

M. tuberculosis complex

   M. bovis BCGe

1

(3), 4, 7

M. tuberculosis complex

M. tuberculosis complex

   M. avium

13

5, 7

MAIS group

M. avium

   M. intracellulare

5

5, 7

MAIS group

M. intracellulare

   M. kansasii

3

3, 7

M. kansasii

M. kansasii

   M. abscessus

1

1, 7

M. chelonae/M. abscessus complex

M. abscessus

   M. chelonae

1

1, 7

M. chelonae/M. abscessus complex

M. chelonae

   M. gordonae

9

2, 7

M. gordonae

M. gordonae

   M. fortuitum

9

6, 7

M. fortuitum

M. fortuitum

   M. lentiflavum

2

7

Mycobacterium spp.

Mycobacterium spp.

   M. marinum

1

3, 7

M. kansasii

M. marinum

   M. xenopi

4

7

Mycobacterium spp.

M. xenopi

Khartoum, Sudan

48

   

   M. tuberculosis e

16

(3), 4, 7

M. tuberculosis complex

M. tuberculosis complex

   M. tuberculosis e + M. fortuitum

7

(3), 4, 6, 7

M. tuberculosis complex + M. fortuitum

M. tuberculosis complex + M. fortuitum

   M. fortuitum

25

6, 7

M. fortuitum

M. fortuitum

TOTAL

242

   
  1. aDifferentiation to species level by use of Genotype CM, Genotype AS, Genotype MTBC or sequencing (16S rDNA or hsp65 gene).
  2. bNumbers in brackets indicate weaker staining.
  3. cMAIS group: M. avium M. intracellulare M. scrofulaceum group.
  4. dIdentification results by Genotype CM are shown. n.d.: not determined.
  5. eAll MTBC isolates yielded weak TL3 signals in addition to TL4
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BMC Infectious Diseases

ISSN: 1471-2334

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