Table 1 Primer sets for HBoV genome sequencing
Primers | Sequences | Amplification position (bases) | Product length |
|---|---|---|---|
VF1 | 5'-GCCGGCAGACATATTGGA-3' | 1-1460 | 1460bp |
VR1 | 5'-TGACCAACGGCTAGAGGATTA-3' | ||
VF2 | 5'-ATGCTAAATCATCCTGTG-3' | 638-2435 | 1798bp |
VR2 | 5'-TTGTCTTTCATATTCCCT-3' | ||
VF3 | 5'-ACCCAAGAAACGTCGTCTAACTG-3' | 2300-4226 | 1927bp |
VR3 | 5'-TTAGTCCAGGAGGAATGTATGCT-3' | ||
VF4 | 5'-GGAGGCAATGCTACAGAAA-3' | 4064-5299 | 1236bp |
VR4 | 5'-TGTACAACAACAACACATTAAAAG-3' |