Alignment of HBV genes. Alignment of the amino acid (aa) sequences of the HBV polymerase (A) and surface (B) genes amplified from samples collected during two episodes of OBI reactivation. The genotype D consensus sequence is shown as a reference in the alignment performed by HIV-GRADE_HBV-tool. Dashes indicate identity to the reference, points indicate sequence gaps due to primer design. Substitutions and ambiguities indicating presence of quasispecies in the direct sequence are shown. Differences between the two sequences are in bold. * = stop codon; aa numbered according to Stuyver et al . The two sequences have been submitted to the GenBank and assigned accession no. JF827279 (SL05_2001), JF827280 (SL02_2010).