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Table 2 Representative M. leprae recombinant antigens examined by comparative genomics and actual immune responses.

From: Immunologically reactive M. leprae antigens with relevance to diagnosis and vaccine development

   % Amino acid identity and orthologous genes 1 Number of Predicted epitopes2 Immunogenicity specificity 3
Gene Predicted Function Size (aa) M. ulcerans M. avium M. bovis(BCG) M. marinum M. tuberculosis T cell B cell WBA IFNγ Ab
ML0405 hypothetical protein 394 29 (MUL_5045) - 62 (BCG_3680c) 57 (MMAR_4515) 62 (Rv3616c) 7 39 +/+ +/+
ML2331 possible secreted protein 256 81 (MUL_4308) 80 (MAV_0385) 82 (BCG_3777) 81 (MMAR_5233) 81 (Rv3717) 4 24 +/+ +/+
ML2055 fibronectin attachment protein 287 65 (MUL_3017) 57 (MAV_2859) 67 (BCG_1896) 65 (MMAR_2737) 67(Rv1860) 6 15 +/+ +/+
ML1685 3-isopropylmalate dehydratase 476 86 (MUL_1968) 88 (MAV_3838) 88 (BCG_3009c) 88 (MMAR_1726) 88 (Rv2988c) 3 25 +/+ -
ML1632 possible hydrolase 511 73 (MUL_1334) 77 (MAV_2243) 75 (BCG_2040c) 74 (MMAR_3295) 75 (Rv2223c) 4 32 +/+ -
ML1556 initiation factor IF-2 924 83 (MUL_2122) 81 (MAV_3693) 83 (BCG_2859c) 82 (MMAR_1894) 83 (Rv2839c) 3 17 +/+ -
ML2044 Possible glycosyl hydrolase (pseudogene) 73 - - - - - 3 7 +/+ -
ML0840 hypothetical protein 434 - 66 (MAV_2053) - - - 3 22 +/+ -
ML0276 hypothetical protein 147 - 71 (MAV_4774) 78 (BCG_0427) 70 (MMAR_0687) 78 (Rv0390) 5 14 +/+ +/-
ML2541 acyl-CoA synthase (pseudogene) 146 77 (MUL_0004) 78 (MAV_0004) 83 (BCG_0004) 78 (MMAR_0004) 77 (Rv0004) 5 15 +/- -
ML2203 hypothetical protein 373 76 (MUL_0420) 76 (MAV_0750) 77 (BCG_0863c) 75 (MMAR_4878) 77 (Rv0811c) 6 17 +/- -
ML2358 probable acyl-CoA synthase 583 77 (MUL_2020) 56 (MAV_1328) 77 (BCG_2952) 77 (MMAR_1777) 76 (Rv2930), 3 15 +/- -
ML2346 hypothetical protein 301 - - - - - 5 12 +/- -
ML2380 possible secreted protein 153 65 (MUL_1413) 66 (MAV_4695) 66 (BCG_0494c) 65 (MMAR_0777) 66 (Rv0455c) 3 15 +/- -
ML2603 possible lysophospholipase 279 73 (MUL_1077) 77 (MAV_4997) 74 (BCG_0220) 74 (MMAR_0427) 74 (Rv0183) 5 16 +/- -
ML0022 hypothetical protein 488 71 (MUL_0024) 66 (MAV_0024) 72 (BCG_0050c) 69 (MMAR_0022) 72 (Rv0020) 5 17 +/- -
  1. 1 Blast Search for amino acid identity was performed using BLAST Uniprot (http://www.uniprot.org/). (-) = no homologue found. Locus_tag othologous genes are indicated within parentheses.
  2. 2Promiscuous T cell epitopes predicted to be recognized by least 26 of 51 different HLA-DR alleles identified by PROPED server (http://www.imtech.res.in/raghava/propred/), and the number of potential B cell epitopes predicted by ABCpred server (http://www.imtech.res.in/raghava/abcpred/ABC_submission.html).
  3. 3Immu/spec = immunogenicity/specificity; WBA +/+ = immunogenic protein that gives specific cellular response; WBA +/- = immunogenic protein that gives unspecific cellular response; Ab +/+: immunogenic protein that induces specific antibody response; Ab+/-: immunogenic protein that induces unspecific antibody response. (-) = not immunogenic.