Identification of leprosy-specific antigens by cellular and antibody responses. WBA-IFNγ secretion and serum IgG responses were determined for ML0405, ML2331 and ML2055. For WBA blood from leprosy patients and controls were incubated for 24 h with ML0405, ML2331 and ML2055 antigens, and IFNγ secretion determined by ELISA. The dotted lines represent the arbitrary cut-off IFNγ above 50 pg/ml. WBA results are presented as box plots encompassing the 25th and 75th percentiles, and the black line within each box indicating the median value, n = 20 per group. IgG ELISA data are shown in scatter plots of individual values for each individual serum tested, n = 45 per group. The dotted line indicates the calculated cut-off for positive responses (OD >0.3).