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Table 2 pncA gene mutation analysis, PZase activity test, and spligotyping of the MDRTB isolates

From: Characteristics of pncA mutations in multidrug-resistant tuberculosis in Taiwan

Mutation site No. of isolates Nucleotide substitution Amino acid change PZA susceptibility PZase activity Spoligotyping
7 1 G→T Ala(3)→Ser Resistant + Beijing
68 1 G→C Gly(23)→Ala Resistant + H3
83 1 C→T Ala(28)→Val Resistant + T1
98 1 A→C Asp(33)→Ala Sensitive + Beijing
134 1 T→G Val(45)→Gly Resistant - Beijing
135 1 T→C Val(45)→Ala Resistant - Orphan strain
161 1 C→T Pro(54)→Leu Resistant + Orphan strain
165 1a T→A Gly(55)→Gly Resistant - Beijing
166 1a G→T Gly(55)→Gly Resistant - Beijing
167 1a A→G Asp(56)→Cys Resistant - Beijing
170 1a A→C His(57)→Pro Resistant - Beijing
171 1, 1a C→T His(57)→Pro Resistant 1+, 1- Beijing
174 1a C→A Phe(58)→Leu Resistant - Beijing
168 1 Ins C Frameshift Resistant - Beijing
185 1 C→A Pro(62)→Gln Resistant + Beijing
211 1 C→T His(71)→Tyr Resistant - Beijing
225 1 A→G Thr(76)→Ala Resistant - Orphan strain
233 1 G→T Gly(78)→Gly Resistant - Beijing
248 1 C→G Pro(83)→Arg Resistant + Orphan strain
254 1 T→C Leu(85)→Pro Resistant - Beijing
290 1 G→A Gly(97)→Asp Resistant - Beijing
298 1b A→G Thr(100)→Ala Resistant - Orphan strain
442 1b C→T Arg(148)→Cys Resistant - Orphan strain
308 1 A→G Tyr(103)→Cys Resistant - Beijing
207-209 1c del ACC - Resistant - Orphan strain
319 1c G→T Glu(107)→Glu Resistant - Orphan strain
403 1c A→G Thr(135)→Ala Resistant - Orphan strain
461 1c G→C Arg(154)→Thr Resistant - Orphan strain
335 2 A→T Asn(112)→Tyr Resistant 1+, 1- Beijing, T2
364 1 C→T Gln(122)→Gln Resistant - Beijing
394 1 G→A Gly(132)→Ser Resistant + Orphan strain
395 1 G→A Gly(132)→Asp Resistant - Orphan strain
401 1 C→T Ala(134)→Val Resistant - Beijing
416 2 T→C Val(139)→Ala Resistant 1+, 1- Beijing, U
529 1 A→C Thr(177)→Pro Resistant + Orphan strain
551 1 G→A Csy(184)→Tyr Resistant - Beijing
- 7 WT WT Resistant 4+, 3- Beijing, Orphan strain
- 29 WT WT Sensitive 27+, 2- Beijing, H3, Orphan strain, T1, T2-T3
  1. a, b, c Multiple mutations within one isolate
  2. WT: wild type