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Table 2 Characterisation of vaginal Lactobacillus strains

From: Extended antimicrobial treatment of bacterial vaginosis combined with human lactobacilli to find the best treatment and minimize the risk of relapses

PCR type strain Species Doubling timea Autoaggregationb H2O2 c Hemolysis Plasmide Antibiotic resistancef Total scoreg
23B33 L. crispatus 92 (1) 3 3 α - MZH, FX 7*
21M49 L. crispatus 91 (1) 3 2 α - MZH 6*
8R6 L. crispatus 83 (2) 3 0 α - MZH 5*
4R5 L. crispatus 109 (1) 3 0 α - MZH 4*
9M9 L. crispatus 102 (1) 1 2 α - MZH 4
13B2 L. crispatus 96 (1) 2 1 α + MZH 0
16B2 L. crispatus 113 (1) 2 1 α + MZH 0
18R5 L. crispatus 95 (1) 2 3 ? d - MZH, FX 0
6M9 L. gasseri 69 (2) 3 1 α - MZH, FX 6*
6M8 L. gasseri 71 (2) 3 0 α - MZH, FX 5*
20M39 L. gasseri 68 (2) 0 1 α - MZH, FX 3*
20M37 L. gasseri 71 (2) 0 1 α - MZH, FX 3
16B1 L. gasseri 122 (0) 1 1 α - MZH, FX 2
7B3 L. gasseri 183 (0) 0 1 α - MZH 1
5M8 L. gasseri 77 (2) 3 1 α + MZH, FX 0
15M7 L. gasseri 68 (2) 1 0 α + MZH, FX 0
22B42 L. jensenii 85 (2) 3 2 α - MZH, FX 7*
12B1 L. jensenii 71 (2) 1 2 α - MZH, FX 5*
  1. a Doubling time: more than 120 min (score 0), between 90-120 min (score 1), between 60-90 min (score 2)
  2. bAutoaggregation: no aggregation (score 0), presence of flakes (score 1), sedimented flakes but cloudy supernatant (score 2), sedimented flakes and clear supernatant (score 3).
  3. c H2O2 production: strongly positive (score 3), intermediate positive (score 2) weakly positive (score 1), or negative (score 0) according to the intensity of blue colour development.
  4. d Slight lysis of human blood cells were observed for 18R5 when grown in anaerobiosis.
  5. e Presence (+) or absence (-) of plasmid
  6. f Antibiotics to which the Lactobacillus strains are resistant among the 10 tested. Metronidazole (MHZ), cefoxitin (FX).
  7. g Total score for a Lactobacillus strain is the sum of scores for doubling time, auto-aggregation and hydrogen peroxide production (max. value 8). Strains showing slight hemolysis (18R5) and presence of plasmid (5M8, 13B2, 15M7, 16B2) were scored 0.
  8. *Strains with the highest score selected for the clinical trial.