IgG antibodies specific to KMP11, HSP70, PFR2 and Tgp63 parasite recombinant antigens. The level of IgG antibodies was measured by ELISA. Data are expressed as optical density (O.D.) values. Statistically significant differences (p < 0.05) are indicated. O.D. values of the percentage of patients comprised between 25% and 75% are represented as vertical boxes and the corresponding medians as a horizontal line inside the boxes. Error bars represent 10% and 90% intervals (A) Sera from 22 healthy donors (HD), 46 non-treated chagasic patients (Ch), 15 patients with autoimmune disease (AI) and 24 patients with an infectious disease other than Chagas disease (Inf). (B) Sera from 22 healthy donors (HD), 15 indeterminate (IND), 16 cardiac (CCC) and 15 digestive (DIG) chagasic patients.