Liver biopsy from a patient with hepatosplenic candidiasis without culture confirmation. Sequencing of PCR amplicons revealed C. tropicalis. Probe Candida 317-FITC (d-f) shows hybridisation, in contrast to probe Asp-F-FITC (a-c). DAPI channel (first column), FITC-channel (second column), Fusion picture DAPI and FITC channels (last column). Note that there is substantial autofluorescence in the FITC channel.