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Figure 5 | BMC Infectious Diseases

Figure 5

From: Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens

Figure 5

Liver biopsy from a patient with hepatosplenic candidiasis without culture confirmation. Sequencing of PCR amplicons revealed C. tropicalis. Probe Candida 317-FITC (d-f) shows hybridisation, in contrast to probe Asp-F-FITC (a-c). DAPI channel (first column), FITC-channel (second column), Fusion picture DAPI and FITC channels (last column). Note that there is substantial autofluorescence in the FITC channel.

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