Figure 5
The detection sensitivity of LAMP assay for EV71. (5A) The electrophoresis diagram of RT-LAMP with virus serial dilution. M: Marker, 100-bp DNA ladder (Sigma, USA). N: negative control without target DNA. Lanes 1-8 are target DNA at 105, 104, 103, 102, 10, 1, 0.1, and 0 PFU/ml, respectively. (5B) Goldview staining of serial dilution reaction. N: Negative. Lanes 1-7; target DNA at 105, 104, 103, 102, 10, 1 and 0.1 PFU/ml, respectively.