Multiplex | Virus Target | Target Gene | Forward primer sequence | Reverse primer sequence | Probe sequence |
---|---|---|---|---|---|
1 | Influenza B | NS | ATGATCTTACAGTGGAGGATGAAGAA | CGAATTGGCTTTGRATGTCCTT | CY5-ATGGCCATCGGATCCTCAAYTCACTCT-BHQ |
 | Influenza C | Matrix | GGCAAGCGACATGCTGAAYA | TCCAGCTGCYTTCATTTGCTTT | VIC-CTCTTCCTTCTGATTTTTTCAAA-MGBNFQ |
2 | Mycoplasma pneumoniae | Cytadhesin P1 (P1) gene | AAGCAGGAGTGACGGAAACAC | CACCACATCATTCCCCGTATT | CY5-CTCCACCAACAACCTCGCGCCTA-BHQ |
 | Human metapneumovirus A | Fusion | GCYGTYAGCTTCAGTCAATTCAA | TCCAGCATTGTCTGAAAATTGC | VIC-CAACATTTAGAAACCTTCT-MGBNFQ |
 | Human metapneumovirus B | Fusion | GCYGTYAGCTTCAGTCAATTCAA (Common with A) | GTTATCCCTGCATTGTCTGAAAACT | VIC-CGCACAACATTTAGGAATCTTCT-MGBNFQ |
 | Parainfluenza virus 1 | HN | GTGATTTAAACCCGGTAATTTCTCA | CCTTGTTCCTGCAGCTATTACAGA | FAM-ACCTATGACATCAACGAC-MGBNFQ |
3 | Parainfluenza virus 2 | HN | ATGAAAACCATTTACCTAAGTGATGGA | CCTCCYGGTATRGCAGTGACTGAAC | VIC-TCAATCGCAAAAGC-MGBNFQ |
 | Parainfluenza virus 3 | HN | CCAGGGATATAYTAYAAAGGCAAAA | CCGGGRCACCCAGTTGTG | FAM-TGGRTGTTCAAGACCTCCATAYCCGAGAAA-BHQ |
 | Parainfluenza virus 4 | Fusion | CAGAYAACATCAATCGCCTTACAAA | TGTACCTATGACTGCCCCAAARA | CY5-CCMATCACAAGCTCAGAAATYCAAAGTCGT-BHQ3A |
4 | Human coronavirus 229E | Nucleocapsid | CAGTCAAATGGGCTGATGCA | AAAGGGCTATAAAGAGAATAAGGTATTCT | FAM-CCCTGACGACCACGTTGTGGTTCA- BHQ |
 | Human coronavirus OC43 | Nucleocapsid | CGATGAGGCTATTCCGACTAGGT | CCTTCCTGAGCCTTCAATATAGTAACC | CY5-TCCGCCTGGCACGGTACTCCCT- BHQ |
 | Human coronavirus NL63 | 1a gene | ACGTACTTCTATTATGAAGCATGATATTAA | AGCAGATCTAATGTTATACTTAAAACTACG | VIC-ATTGCCAAGGCTCCTAAACGTACAGGTGTT-TAMRA |
5 | Respiratory syncitial virus A | NP | AGATCAACTTCTGTCATCCAGCAA | TTCTGCACATCATAATTAGGAG | FAM-CACCATCCAACGGAGCACAGGAGAT-BHQ |
 | Respiratory syncitial virus B | NP | AAGATGCAAATCATAAATTCACAGGA | TGATATCCAGCATCTTTAAGTA | FAM-TTTCCCTTCCTAACCTGGACATA-BHQ |
 | Rhinovirus | 5'-UTR | TGGACAGGGTGTGAAGAGC | CAAAGTAGTCGGTCCCATCC | VIC-TCCTCCGGCCCCTGAATG-TAMRA |
 | Adenovirus | Matrix | GCCACGGTGGGGTTTCTAAACTT | GCCCCAGTGGTCTTACATGCACATC | CY5-TGCACCAGACCCGGGCTCAGGTACTCCGA-BHQ |