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Table 3 Multiplex design

From: Sequential multiplex PCR assay for determining capsular serotypes of colonizing S. pneumoniae

Primers Primer concentration (μM)
Reaction 1 6A/B/C 1
  23F 1.5
  11A 1.5
  23A 2
Reaction 2 23B 0.75
  19F 1
  19A 1.5
  38 1.5
Reaction 3 1 0.75
  3 1
  15B/C 1.25
  35B 1.5
Reaction 4 14 0.75
  34 0.75
  22F 1.25
  9A/V 1.5
Reaction 5 35A 0.75
  12F 1
  24A/F 1.25
  16F 1.25
  7A/F 2
Reaction 6 33F 1
  4 1
  35F 1.25
  10A 1.5
  31 1.5
Reaction 7 7B/C 0.75
  15A 1
  18C 1
  17F 1.25
  1. Primers cpsA-f and cpsA-r were included in all reactions at 0.5 μM each