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Table 3 Multiplex design

From: Sequential multiplex PCR assay for determining capsular serotypes of colonizing S. pneumoniae

Primers

Primer concentration (μM)

Reaction 1

6A/B/C

1

 

23F

1.5

 

11A

1.5

 

23A

2

Reaction 2

23B

0.75

 

19F

1

 

19A

1.5

 

38

1.5

Reaction 3

1

0.75

 

3

1

 

15B/C

1.25

 

35B

1.5

Reaction 4

14

0.75

 

34

0.75

 

22F

1.25

 

9A/V

1.5

Reaction 5

35A

0.75

 

12F

1

 

24A/F

1.25

 

16F

1.25

 

7A/F

2

Reaction 6

33F

1

 

4

1

 

35F

1.25

 

10A

1.5

 

31

1.5

Reaction 7

7B/C

0.75

 

15A

1

 

18C

1

 

17F

1.25

  1. Primers cpsA-f and cpsA-r were included in all reactions at 0.5 μM each
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BMC Infectious Diseases

ISSN: 1471-2334

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