Analysis of GSK3β and CDK5 in the brain tissues of normal and scrapie-infected hamsters collected at the moribund stage. A. Western blots. Same amounts of individual brain homogenate were loaded in 12% SDS-PAGE. The scrapie agents and normal controls were shown on the top of the graphs. Various specific immunoblots were indicated by arrows on the left. B. Quantitative analysis of each gray numerical value of GSK3β and CDK5 vs that of β-actin. The average values were calculated from five individual infected hamsters or four individual normal hamsters and presented as mean ± SD. Statistical differences compared with controls were illustrated as P < 0.05 and P < 0.01. C. Real time PCR analysis of the mRNA transcription of GSK3β and CDK5. The transcription level of each specific mRNA was determined relative to that of the individual β-actin. The relative intensity of each gene from scrapie-infected hamsters was relative to that of respective gene from normal hamsters that was set to 1. Data are representative of three independent experiments.