Western blot analysis of PrP
in the brain tissues of scrapie agents 263 K- and 139A-infected hamsters collected at the moribund stage. For detection of PrPSc, all tested brain homogenates were digested with a final concentration of 50 μg/ml PK at 37°C for 60 min. Same amounts of individual brain homogenate were loaded in 15% SDS-PAGE. The scrapie agents were shown on the top of the graphs. PrPSc specific immunoblots (non-, mono- and di-glycosylated PrP isoforms) were indicated by arrows on the left and molecular mass markers were indicated to the right.