Primer | Sequence (5'-3') | Product size (bp) | PCR conditions |
---|---|---|---|
icaA forward icaA reverse | GAC CTC GAA GTC AAT AGA GGT CCC AGT ATA ACG TTG GAT ACC | 814 | 30 s 95°C 60 s 60°C 90 s 72°C |
icaB forward icaB reverse | ATG GCT TAA AGC ACA CGA CGC TAT CGG CAT CTG GTG TGA CAG | 526 | 60 s 95°C 60 s 59°C 90 s 72°C |
icaC forward icaC reverse | ATA AAC TTG AAT TAG TGT ATT ATA TAT AAA ACT CTC TTA ACA | 989 | 30 s 95°C 60 s 42°C 90 s 72°C |
IS256 forward IS256 reverse | TGA AAA GCG AAG AGA TTC AAA GC ATG TAG GTC CAT AAG AAC GGC | 1102 | 60 s 94°C 60 s 59°C 90 s 68°C |
icaD forward icaD reverse | AGG CAA TAT CCA ACG GTA A GTC ACG ACC TTT CTT ATA TT | 371 | 60 s 94°C 60 s 59°C 150 s 72°C |