Skip to main content

Table 1 Primer sequences for PCR used in this study.

From: Production of icaADBC-encoded polysaccharide intercellular adhesin and therapeutic failure in pediatric patients with staphylococcal device-related infections

Primer

Sequence (5'-3')

Product size (bp)

PCR conditions

icaA forward

icaA reverse

GAC CTC GAA GTC AAT AGA GGT

CCC AGT ATA ACG TTG GAT ACC

814

30 s 95°C

60 s 60°C

90 s 72°C

icaB forward

icaB reverse

ATG GCT TAA AGC ACA CGA CGC

TAT CGG CAT CTG GTG TGA CAG

526

60 s 95°C

60 s 59°C

90 s 72°C

icaC forward

icaC reverse

ATA AAC TTG AAT TAG TGT ATT

ATA TAT AAA ACT CTC TTA ACA

989

30 s 95°C

60 s 42°C

90 s 72°C

IS256 forward

IS256 reverse

TGA AAA GCG AAG AGA TTC AAA GC

ATG TAG GTC CAT AAG AAC GGC

1102

60 s 94°C

60 s 59°C

90 s 68°C

icaD forward

icaD reverse

AGG CAA TAT CCA ACG GTA A

GTC ACG ACC TTT CTT ATA TT

371

60 s 94°C

60 s 59°C

150 s 72°C