Cloning and Expression of N-terminal fragment (203aa) of P116 protein of
M. pneumoniae. A: Schematic diagram showing P116 gene sequence and location of fragment of P116 gene selected for cloning and expression. Fig. also shows positions of F1 (forward) and R1 (reverse) primers within the gene coding for P116 protein. B: SDS-PAGE analysis of N-terminal P116 protein showing its expression in E. coli and its subcellular localization. Lane1: Prestained standard protein marker, Lane 2: Protein extracts of uninduced E. coli, Lane 3: Protein extracts of induced (~27 KDa) E. coli, Lanes 4&5: E. coli pellets of the expressed protein after sonication, Lanes 6&7: Supernatant after sonication. C: Immunoblot analysis of crude P116 protein with anti. His antibodies-Lane1: Prestained standard protein marker, Lane 2: Protein extracts of uninduced E. coli, Lane 3: Recombinant protein detected with anti. His antibodies.