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Table 3 MIRU-VNTR genotypes of M. tuberculosis isolates from patients with multiple strain TB infections

From: Detection of multiple strains of Mycobacterium tuberculosis using MIRU-VNTR in patients with pulmonary tuberculosis in Kampala, Uganda

   

MIRU-VNTR Locus amplified

PTID

Time point

Sample

580

960

1644

2996

3192

802

424

577

1955

2163

2165

2401

3690

4052

4156

A

BL

35357

2,5

2

4,5

3,4,6

1,3,5

3,4,6

 

4

2,6

1,3

4

1,2

3

2,6

5

 

BL

35358

2,5

2

4,5

4

1,3

4

 

4

2

1

 

1

 

2

 

B

BL

33007

2

5

1,3,4

4,6

4

4,6,7

5

2

 

1,2,3

3

2,3,5

 

4

 

C

BL

34347

2

5

1

3

2

4

 

5

4

2

2

2

 

3

 
 

BL

34348

2

5

1

3

2,3

3,4,5

 

5,4,3

2,3,4

2

2,3,4

2,3,4

 

3

 

D

BL

33914

2

4

4

4

2

3

4

3

3

2

4

4

4

2

3

 

BL

33913

2

4

4

3

1

2

 

4

3

2

4

2

 

2

3

E

BL

35584

2

2

3

3

3

4

2

4

3

2

3

2

2

3

8

 

BL

35583

3

4

5

3

2

3

2

4

2

2

4

2

4

3

3

F

BL

37768

2

1

1

2

1

2

 

4

2

2

2

2

2

3

 
 

BL

37767

2

5

1

3

1

3

4

5

2

1

2

2

2

3

 

G

BL

38228

2

7

3

6,8

5

4

 

2

2,3,4

4

3

6

 

4

9

H

BL

33027

MOTT

              
 

BL

33026

MOTT

              
 

M2

33608

2'

5

3

6

4

6

5

2

 

2

3

3

 

4

 
 

M2

33609

2

5

3

4,6

  

5

2,3

 

2

3

2, 3

 

4

 
 

M2

33610

2'

5

1

6

 

4

5

2

 

2

3

3

 

4

 
  1. The numerical figures (1 to 9) represent the number of alleles per amplified MIRU-VNTR loci, as described by Supply et al [19, 20]. Where there is more than one numerical figure per locus implies existence of more than one MTB strain in the sample, indicating multiple strain infection with M. tuberculosis (Patient samples A, B, C, and G). Serial samples from patients D, E, F, and H had different strains at different sampling points, which also indicate multiple strains infection. For patients A-G, blanks in copy numbers are presumed to be due to technical laboratory error. For patient H, blanks are likely due to MOTT co-infection.
  2. 2' is a standard copy number noted in the MIRU manual; PTID, Patient Identification; BL, Baseline sample collection; M2, sample collection in the second month; MOTT, Mycobcateria Other Than Tuberculosis