| | | |
MIRU-VNTR Locus amplified
|
|---|
|
PTID
|
Time point
|
Sample
|
580
|
960
|
1644
|
2996
|
3192
|
802
|
424
|
577
|
1955
|
2163
|
2165
|
2401
|
3690
|
4052
|
4156
|
|---|
|
A
|
BL
|
35357
|
2,5
|
2
|
4,5
|
3,4,6
|
1,3,5
|
3,4,6
| |
4
|
2,6
|
1,3
|
4
|
1,2
|
3
|
2,6
|
5
|
| |
BL
|
35358
|
2,5
|
2
|
4,5
|
4
|
1,3
|
4
| |
4
|
2
|
1
| |
1
| |
2
| |
|
B
|
BL
|
33007
|
2
|
5
|
1,3,4
|
4,6
|
4
|
4,6,7
|
5
|
2
| |
1,2,3
|
3
|
2,3,5
| |
4
| |
|
C
|
BL
|
34347
|
2
|
5
|
1
|
3
|
2
|
4
| |
5
|
4
|
2
|
2
|
2
| |
3
| |
| |
BL
|
34348
|
2
|
5
|
1
|
3
|
2,3
|
3,4,5
| |
5,4,3
|
2,3,4
|
2
|
2,3,4
|
2,3,4
| |
3
| |
|
D
|
BL
|
33914
|
2
|
4
|
4
|
4
|
2
|
3
|
4
|
3
|
3
|
2
|
4
|
4
|
4
|
2
|
3
|
| |
BL
|
33913
|
2
|
4
|
4
|
3
|
1
|
2
| |
4
|
3
|
2
|
4
|
2
| |
2
|
3
|
|
E
|
BL
|
35584
|
2
|
2
|
3
|
3
|
3
|
4
|
2
|
4
|
3
|
2
|
3
|
2
|
2
|
3
|
8
|
| |
BL
|
35583
|
3
|
4
|
5
|
3
|
2
|
3
|
2
|
4
|
2
|
2
|
4
|
2
|
4
|
3
|
3
|
|
F
|
BL
|
37768
|
2
|
1
|
1
|
2
|
1
|
2
| |
4
|
2
|
2
|
2
|
2
|
2
|
3
| |
| |
BL
|
37767
|
2
|
5
|
1
|
3
|
1
|
3
|
4
|
5
|
2
|
1
|
2
|
2
|
2
|
3
| |
|
G
|
BL
|
38228
|
2
|
7
|
3
|
6,8
|
5
|
4
| |
2
|
2,3,4
|
4
|
3
|
6
| |
4
|
9
|
|
H
|
BL
|
33027
|
MOTT
| | | | | | | | | | | | | | |
| |
BL
|
33026
|
MOTT
| | | | | | | | | | | | | | |
| |
M2
|
33608
|
2'
|
5
|
3
|
6
|
4
|
6
|
5
|
2
| |
2
|
3
|
3
| |
4
| |
| |
M2
|
33609
|
2
|
5
|
3
|
4,6
| | |
5
|
2,3
| |
2
|
3
|
2, 3
| |
4
| |
| |
M2
|
33610
|
2'
|
5
|
1
|
6
| |
4
|
5
|
2
| |
2
|
3
|
3
| |
4
| |
- The numerical figures (1 to 9) represent the number of alleles per amplified MIRU-VNTR loci, as described by Supply et al [19, 20]. Where there is more than one numerical figure per locus implies existence of more than one MTB strain in the sample, indicating multiple strain infection with M. tuberculosis (Patient samples A, B, C, and G). Serial samples from patients D, E, F, and H had different strains at different sampling points, which also indicate multiple strains infection. For patients A-G, blanks in copy numbers are presumed to be due to technical laboratory error. For patient H, blanks are likely due to MOTT co-infection.
- 2' is a standard copy number noted in the MIRU manual; PTID, Patient Identification; BL, Baseline sample collection; M2, sample collection in the second month; MOTT, Mycobcateria Other Than Tuberculosis
