Detection of multiple strains of Mycobacterium tuberculosis using MIRU-VNTR in patients with pulmonary tuberculosis in Kampala, Uganda

Table 1 PCR primer sequences and MIRU-VNTR locus designations1 used in this study

Locus	Alias	Repeat unit length, bp	PCR primer pairs (5'-3')
580	MIRU4, ETRD	77	GCGCGAGAGCCCGAACTGC GCGCAGCAGAAACGCCAGC
2996	MIRU26	51	TAGGTCTACCGTCGAAATCTGTGAC CATAGGCGACCAGGCGAATAG
802	MIRU40	54	GGGTTGCTGGATGACAACGTGT GGGTGATCTCGGCGAAATCAGATA
960	MIRU10	53	GTTCTTGACCAACTGCAGTCGTCC GCCACCTTGGTGATCAGCTACCT
1644	MIRU16	53	TCGGTGATCGGGTCCAGTCCAAGTA CCCGTCGTGCAGCCCTGGTAC
3192	MIRU31, ETRE	53	ACTGATTGGCTTCATACGGCTTTA GTGCCGACGTGGTCTTGAT
424	Mtub04	51	CTTGGCCGGCATCAAGCGCATTATT GGCAGCAGAGCCCGGGATTCTTC
577	ETR C	58	CGAGAGTGGCAGTGGCGGTTATCT AATGACTTGAACGCGCAAATTGTGA
2165	ETR A	75	AAATCGGTCCCATCACCTTCTTAT CGAAGCCTGGGGTGCCCGCGATTT
2401	Mtub30	58	CTTGAAGCCCCGGTCTCATCTGT ACTTGAACCCCCACGCCCATTAGTA
3690	Mtub39	58	CGGTGGAGGCGATGAACGTCTTC TAGAGCGGCACGGGGGAAAGCTTAG
4156	QUB-4156	59	TGACCACGGATTGCTCTAGT GCCGGCGTCCATGTT
2163b	QUB-11b	69	CGTAAGGGGGATGCGGGAAATAGG CGAAGTGAATGGTGGCAT
1955	Mtub21	57	AGATCCCAGTTGTCGTCGTC CAACATCGCCTGGTTCTGTA
4052	QUB-26	111	AACGCTCAGCTGTCGGAT CGGCCGTGCCGGCCAGGTCCTTCCCGAT

¹: Extracted from Supply et al, [19]. Primers were synthesized by Eurofins MWG Operon, Ebersberg (Germany).

ISSN: 1471-2334