Table 1 Specific T. denticol a primers sets and cycling condition used in this study.
| Primer | Nucleotide Sequence | Amplicon Size | Cycling conditions |
|---|---|---|---|
|
Dent 1 Dent 2 |
5'TAATACCGAATGTGCTCATTTACAT3' 5'TCAAAGAAGCATTCCCTCTTCTTCTTA3' | 316 bp |
36 cycles 95°C 30" 60°C 1' 72°C 1' |
|
Kx14 Kx04 |
5'GCTTGACAAGTGGATTTGGCTGTG3' 5'GAGAATAGCAGCAGAGTCTATTAG3' | 1777 bp |
30 cycles 94°C 1' 55°C 1' 72°C 3' |
|
Kx14 Kx09 |
5'GCTTGACAAGTGGATTTGGCTGTG3' 5'CGAACGTCACCTTCGGTCTTTGAG3' | 294 bp |
31 cycles 94°C 1' 55°C 1' 72°C 1' |
|
Td03 Td06 |
5'CTCAAAGACCGAAGGTGACGTTCG3' 5'GCATATTTGTTTGCTGCG3' | 571 bp |
31 cycles 94°C 1' 55°C 1' 72°C 1' |
|
Td05 Kx04 |
5'CCGCAGCAAACAAATATGC3' 5' GAGAATAGCAGCAGAGTCTATTAG3' | 875 bp |
31 cycles 94°C 1' 55°C 1' 72°C 2' |