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Table 1 Specific T. denticol a primers sets and cycling condition used in this study.

From: The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis

Primer

Nucleotide Sequence

Amplicon Size

Cycling conditions

Dent 1

Dent 2

5'TAATACCGAATGTGCTCATTTACAT3'

5'TCAAAGAAGCATTCCCTCTTCTTCTTA3'

316 bp

36 cycles

95°C 30"

60°C 1'

72°C 1'

Kx14

Kx04

5'GCTTGACAAGTGGATTTGGCTGTG3'

5'GAGAATAGCAGCAGAGTCTATTAG3'

1777 bp

30 cycles

94°C 1'

55°C 1'

72°C 3'

Kx14

Kx09

5'GCTTGACAAGTGGATTTGGCTGTG3'

5'CGAACGTCACCTTCGGTCTTTGAG3'

294 bp

31 cycles

94°C 1'

55°C 1'

72°C 1'

Td03

Td06

5'CTCAAAGACCGAAGGTGACGTTCG3'

5'GCATATTTGTTTGCTGCG3'

571 bp

31 cycles

94°C 1'

55°C 1'

72°C 1'

Td05

Kx04

5'CCGCAGCAAACAAATATGC3'

5' GAGAATAGCAGCAGAGTCTATTAG3'

875 bp

31 cycles

94°C 1'

55°C 1'

72°C 2'