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Table 5 Interference of background human DNA in quantitation of HPV-16 DNA with HPV-16 E6 and HPV-16 E2 real-time PCR assays.

From: Human papillomavirus (HPV) types 16, 18, 31, 45 DNA loads and HPV-16 integration in persistent and transient infections in young women

No. of copies of human DNA Quantitation of 1000 copies of episomal HPV-16 DNA with real-time PCR assays
  HPV-16 E2 HPV-16 E6
0 933 ± 13 1150 ± 39
8 × 103 1080 ± 35 1114 ± 7
1 × 104 1097 ± 38 1179 ± 148
2 × 104 1026 ± 149 1109 ± 59
4 × 104 1125 ± 15 1015 ± 46
6 × 104 1173 ± 10 820 ± 30
8 × 104 1151 ± 100 726 ± 19
1 × 105 1082 ± 82 787 ± 32
2 × 105 1071 ± 38 562 ± 20
  1. 1000 copies of HPV-16 DNA were amplified in a background of various copies of human DNA and detected in real-time PCR assays for quantitation of HPV-16 E6 and HPV-16 E2. Results are means ± 1 standard deviation of duplicate values. Repeat experiments gave similar results.