Figure 1

Agarose gel showing amplification of a DNA fragment of ~106 bp by seminested PCR with DNA isolated from serum from patient 1 (lane 2) and patient 2 (lane 3). An amplicon of the same size was also obtained with genomic DNA isolated from reference strain of C. tropicalis (lane 4) while no amplicon was obtained in the reagent control tube in which water instead of DNA was added (lane 1). Lane M is 100 bp DNA marker and the positions of migration of 100 bp, 200 bp and 600 bp fragments are marked.