Skip to main content
Figure 1 | BMC Infectious Diseases

Figure 1

From: Rv1985c, a promising novel antigen for diagnosis of tuberculosis infection from BCG-vaccinated controls

Figure 1

Purification and analysis of recombinant Rv1985c. (A) Electrophoresis analysis on a 12% SDS-PAGE gel stained with Coomassie blue; Lane 1, Takara low molecular weight marker; Lane 2, crude E. colisupernatant fraction before application to column (induced with IPTG); Lane 3, flow-through fraction of His-bind resin; Lane 4, fraction washed with 120 mM imidazole; Lane 5, fraction eluted with 250 mM imidazole. Molecular weight of Rv1985c is approximately 33.8 kDa and protein marker sizes are indicated on the side of gel. (B) Mass-fingerprinting protein assay of 33.9-kDa by MALDITOF.

Back to article page

BMC Infectious Diseases

ISSN: 1471-2334

Contact us