Skip to main content

Table 1 Primers used for the Amplification of WUPyV VP1, KIPyV VP1, and HBoV VP2

From: High prevalence of antibodies against polyomavirus WU, polyomavirus KI, and human bocavirus in German blood donors

Primer

Sequence (5' - 3')a

Gene

Positionb

Virus

WU1673s-TOPO

CACCGCCTGCACAGCAAAGC

VP1

1673-1688

WUPyV

WU2782a

ACATTATCCTTGTGTGTTTAGTATTGGGCC

VP1

2782-2753

WUPyV

KI1501s-TOPO

CACCAGCTGCACCCCGTGT

VP1

1501-1519

KIPyV

KI2727a

CCTTACTGAGTTTGCCACTATGCA

VP1

2727-2704

KIPyV

BoV3446s-TOPO

CACCTCTGACACTGACATTCAAGAC

VP2

3446-3466

HBoV

BoV5113a

AGGAGGAACTTGTAAGCAGAAGC

VP2

5113-5091

HBoV

  1. a Nucleotides added for directional cloning are underlined.
  2. b Positions according to GenBank accession number EU711057 (WUPyV), NC_009238 (KIPyV), and DQ000496 (HBoV).