Confirmation of expression of recombinant proteins. Sf9 cells were infected with recombinant baculoviruses BacWUVP1, BacKIVP1, and BacBoVP2. Uninfected Sf9 cells served as negative control. After SDS-PAGE and immunoblot, proteins were visualised by staining with anti-V5 antibody. The expected protein sizes are ~46 kDa (WUPyV and KIPyV VP1) and ~66 kDa (HBoV VP2).