Table 5 Systematic review of the published studies on prevalence of HPV types in the general population in Italy
| Â | Â | Â | Â | Â | Â | Â | percentage of positive in the population | Â | Â | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Author | pub year | Area | N | Age | Population | type of test | HR+LR | HR | HPV16 | HPV18 | HPV16+18 | Notes on molecular methods | Notes on possible selection biases |
Astori [24] | 1997 | Udine | 197 | mean 37.7 | women referred to gynaecological clinic for genital complaints, Pap negative | PCR My09/11 and RFLP analysis | 20.3 | Â | 5.1 | Â | Â | Â | Â |
Ronco [8] | 2005 | Turin | 1000 | 25-70 | Invited in Organized Screening program | PCR-EIA Gp5+/Gp6+ home made | 8.8 | 7.1 | 2.8 | 0.1 | 2.9 | Â | Â |
Centurioni [6] | 2005 | Genoa | 503 | mean 51 | Opportunistic screening | PCR Dot blot | 15.9 | Â | 9.1 | 5.8 | 14.9 | the method is not actual any more | The recruitment started in 1992 |
Tornesello [25] | 2006 | Milan and Neaples | 207 | median 40 | asymptomatic attending clinic | PCR home made and direct sequence | 19.7 | 16.9 | 8.7 | 0 | 8.7 | Â | Â |
Tornesello [26] | 2007 | Neaples | 31 | mean 28.9 | migrant | PCR nested (MY09-11 follwed by the inner GP5+/6+)followed by sequencing | 35.5 | 25.8 | 6.4 | 0 | 6.4 | Â | Â |
Ammatuna [9] | 2008 | Sicily | 1006 | 18-24 | random population sample | Linear array HPV genotyping test (Roche) | 24.1 | 17.4 | 4.5 | 1.3 | 5.8 | Â | Â |
Tornesello [7] | 2008 | Neaples | 115 | mean 32.5 | Opportunistic screening | Home made Nested PCR followe by direct sequencing | 13.9 | 13.9 | 6.9 | 0.9 | 7.8 | Â | Â |
Del Prete [27] | 2008 | Apulia | 871 | >20; 85% = <50 | women referred to hospital gynecologic visit | PCR E1-E2 followed by gel | 23.1 | 19.4 | 9.6 | 1.6 | 10.1 | Â | Not clear reason for referral, includes women referred for colposcopy |
Agarossi [28] | 2009 | Italy | 9947 | 15->65; 85% <50 | asymptomatic attending clinic | HCII followed by typing with Gp5+/Gp6+ PCR EIA Home made | Â | 14.8 | 4.3 | 1.5 | 5.6 | no mention of negative control for PCR | the sample includes women with previous positive Pap test |
Giovannelli [29] | 2009 | Sicily | 120 | 88% <40 | Migrant attending Clinical visit | PCR-Inno-LiPA | 47.8 | 34.8 | 7.8 | 6 | 13.8 | Â | Â |
Masia [30] | 2009 | Sardinia | 815 | >18; 83% = <45 | Counselling for sexual activity or screening program | PCR-EIA Gp5+/Gp6+ home made | 31 | 20.3 | 8.2 | Â | 13.5 | Â | Â |
Verteramo [31] | 2009 | Rome | 857 | 17-57 | asymptomatic attending clinic | PCR MY09/11 (and PCR with 4 pairs of primers to amplifiy E6 and E7 gene) followed by sequencing | 31 | 13.3 | 4.4 | 0.8 | 5.3 | Â | Â |