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Table 2 Virucidal activity of ethanol, isopropanol and peracetic acid (PAA) against MVA depending on concentration and contact time

From: Can vaccinia virus be replaced by MVA virus for testing virucidal activity of chemical disinfectants?

  MVA reduction factor (RF) (log10)
biocide results lab 1 results lab 2 results lab 3
  1 min 1 min 1 min
30% ethanol -0.10 ± 0.37 -0.06 ± 0.51 -0.14 ± 0.62
40% ethanol 2.80 ± 0. 53 ≥5.32 ± 0.38 1.71 ± 0.60
50% ethanol ≥5.40 ± 0.36 ≥5.32 ± 0.38 ≥3.57 ± 0.40
60% ethanol ≥5.40 ± 0.36 ≥5.32 ± 0.38 ≥3.57 ± 0.40
20% isopropanol 0.06 ± 0.37 -0.44 ± 0.63 0.42 ± 0.46
30% isopropanol 0.50 ± 0.44 3.69 ± 0.53 0.71 ± 0.60
40% isopropanol ≥5.40 ± 0.36 ≥5.32 ± 0.38 ≥3.57 ± 0.40
50% isopropanol ≥5.40 ± 0.36 ≥5.32 ± 0.38 ≥3.57 ± 0.40
60% isopropanol ≥5.40 ± 0.36 ≥5.32 ± 0.38 ≥3.57 ± 0.40
0.001% PAA 0.90 ± 0.47 0.19 ± 0.56 1.14 ± 0.56
0.0025% PAA 4.75 ± 0.31 n.d. n.d.
0.005% PAA ≥5.66 ± 0.47 n.d. n.d.
0.01% PAA ≥5.66 ± 0.47 ≥5.32 ± 0.38 ≥3.57 ± 0.56
0.05% PAA ≥4.60 ± 0.47 ≥4.32 ± 0.38 ≥4.14 ± 0.40
0.1% PAA ≥3.94 ± 0.47 ≥5.19 ± 0.43 ≥4.14 ± 0.40
  1. The prefix "≥" indicates that the determination of the RF is limited by cytotoxic reactions and/or the amount of infectious viruses in the test virus suspension
  2. n.d. = not done