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Table 2 Virucidal activity of ethanol, isopropanol and peracetic acid (PAA) against MVA depending on concentration and contact time

From: Can vaccinia virus be replaced by MVA virus for testing virucidal activity of chemical disinfectants?

 

MVA reduction factor (RF) (log10)

biocide

results lab 1

results lab 2

results lab 3

 

1 min

1 min

1 min

30% ethanol

-0.10 ± 0.37

-0.06 ± 0.51

-0.14 ± 0.62

40% ethanol

2.80 ± 0. 53

≥5.32 ± 0.38

1.71 ± 0.60

50% ethanol

≥5.40 ± 0.36

≥5.32 ± 0.38

≥3.57 ± 0.40

60% ethanol

≥5.40 ± 0.36

≥5.32 ± 0.38

≥3.57 ± 0.40

20% isopropanol

0.06 ± 0.37

-0.44 ± 0.63

0.42 ± 0.46

30% isopropanol

0.50 ± 0.44

3.69 ± 0.53

0.71 ± 0.60

40% isopropanol

≥5.40 ± 0.36

≥5.32 ± 0.38

≥3.57 ± 0.40

50% isopropanol

≥5.40 ± 0.36

≥5.32 ± 0.38

≥3.57 ± 0.40

60% isopropanol

≥5.40 ± 0.36

≥5.32 ± 0.38

≥3.57 ± 0.40

0.001% PAA

0.90 ± 0.47

0.19 ± 0.56

1.14 ± 0.56

0.0025% PAA

4.75 ± 0.31

n.d.

n.d.

0.005% PAA

≥5.66 ± 0.47

n.d.

n.d.

0.01% PAA

≥5.66 ± 0.47

≥5.32 ± 0.38

≥3.57 ± 0.56

0.05% PAA

≥4.60 ± 0.47

≥4.32 ± 0.38

≥4.14 ± 0.40

0.1% PAA

≥3.94 ± 0.47

≥5.19 ± 0.43

≥4.14 ± 0.40

  1. The prefix "≥" indicates that the determination of the RF is limited by cytotoxic reactions and/or the amount of infectious viruses in the test virus suspension
  2. n.d. = not done