Table 1 Virucidal activity of ethanol, isopropanol and peracetic acid (PAA) against VACV depending on concentration and contact time

From: Can vaccinia virus be replaced by MVA virus for testing virucidal activity of chemical disinfectants?

	VACV reduction factor (RF) (log₁₀)
biocide	results lab 1	results lab 2	results lab 3
	1 min	1 min	1 min
30% ethanol	0.13 ± 0.38	-0.25 ± 0.33	≥0.14 ± 0.60
40% ethanol	2.26 ± 0.36	2.69 ± 0.54	3.86 ± 0.48
50% ethanol	≥4.38 ± 0.37	≥5.94 ± 0.31	≥5.0 ± 0.40
60% ethanol	≥4.38 ± 0.37	≥5.94 ± 0.31	≥5.0 ± 0.40
20% isopropanol	0.82 ± 0.27	0.00 ± 0.52	-0.57 ± 0.45
30% isopropanol	0.32 ± 0.34	1.87 ± 0.25	1.86 ± 0.48
40% isopropanol	≥4.38 ± 0.37	≥5.94 ± 0.31	≥5.0 ± 0.40
50% isopropanol	≥4.38 ± 0.37	≥5.94 ± 0.31	≥5.0 ± 0.40
60% isopropanol	≥4.38 ± 0.37	≥5.94 ± 0.31	≥5.0 ± 0.40
0.001% PAA	0.94 ± 0.41	0.56 ± 0.48	0.71 ± 0.53
0.0025% PAA	3.38 ± 0.52	n.d.	n.d.
0.005% PAA	≥4.50 ± 0.38	n.d.	n.d.
0.01% PAA	≥3.50 ± 0.38	≥5.94 ± 0.31	≥4.0 ± 0.40
0.05% PAA	≥3.50 ± 0.38	≥4.94 ± 0.31	≥4.0 ± 0.40
0.1% PAA	≥4.57 ± 0.41	≥6.38 ± 0.37	≥3.86 ± 0.39

The prefix "≥" indicates that the determination of the RF is limited by cytotoxic reactions and/or the amount of infectious viruses in the test virus suspension
n.d. = not done

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