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Table 1 Virucidal activity of ethanol, isopropanol and peracetic acid (PAA) against VACV depending on concentration and contact time

From: Can vaccinia virus be replaced by MVA virus for testing virucidal activity of chemical disinfectants?

  VACV reduction factor (RF) (log10)
biocide results lab 1 results lab 2 results lab 3
  1 min 1 min 1 min
30% ethanol 0.13 ± 0.38 -0.25 ± 0.33 ≥0.14 ± 0.60
40% ethanol 2.26 ± 0.36 2.69 ± 0.54 3.86 ± 0.48
50% ethanol ≥4.38 ± 0.37 ≥5.94 ± 0.31 ≥5.0 ± 0.40
60% ethanol ≥4.38 ± 0.37 ≥5.94 ± 0.31 ≥5.0 ± 0.40
20% isopropanol 0.82 ± 0.27 0.00 ± 0.52 -0.57 ± 0.45
30% isopropanol 0.32 ± 0.34 1.87 ± 0.25 1.86 ± 0.48
40% isopropanol ≥4.38 ± 0.37 ≥5.94 ± 0.31 ≥5.0 ± 0.40
50% isopropanol ≥4.38 ± 0.37 ≥5.94 ± 0.31 ≥5.0 ± 0.40
60% isopropanol ≥4.38 ± 0.37 ≥5.94 ± 0.31 ≥5.0 ± 0.40
0.001% PAA 0.94 ± 0.41 0.56 ± 0.48 0.71 ± 0.53
0.0025% PAA 3.38 ± 0.52 n.d. n.d.
0.005% PAA ≥4.50 ± 0.38 n.d. n.d.
0.01% PAA ≥3.50 ± 0.38 ≥5.94 ± 0.31 ≥4.0 ± 0.40
0.05% PAA ≥3.50 ± 0.38 ≥4.94 ± 0.31 ≥4.0 ± 0.40
0.1% PAA ≥4.57 ± 0.41 ≥6.38 ± 0.37 ≥3.86 ± 0.39
  1. The prefix "≥" indicates that the determination of the RF is limited by cytotoxic reactions and/or the amount of infectious viruses in the test virus suspension
  2. n.d. = not done