Table 1 Strains, plasmids and primers used.
Strains, Plasmids and Primers | Characteristics | Reference or source |
|---|---|---|
Strains | ||
Pseudomonas aeruginosa | ||
PUPa3 | Wild type, rice rhizosphere isolate | [42] |
LASI | lasI::Km of P. aeruginosa PUPa3; Kmr | [43] |
RHLI | rhlI::Gm of P. aeruginosa PUPa3; Gmr | [43] |
DMI | lasI::Km rhlI::Gm of P. aeruginosa PUPa3; Kmr Gmr | [43] |
Pseudomonas putida | ||
SM17 | rsaL::Tc of P. aeruginosa PUPa3; Tcr | [43] |
E. coli | ||
M15(pRep4) | Derivative of E. coli K-12, containing pREP4 plasmid ensuring the production of high levels of lac repressor protein; Kmr | Qiagen |
DH5α | F'/endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 (lacZYA-argF)U169 deoR [80dlac(lacZ)M15recA1] | [44] |
Plasmids | ||
pMP220 | Promoter probe vector, IncP1; Tcr | [45] |
pMOSBlue | Cloning vector; Ampr | Amersham-Pharmacia |
pGEM-T Easy | Cloning vector; Ampr | Promega |
pBBRmcs3 | Broad-host-range vector; Tcr | [46] |
pQE30 | Expression vector, Ampr | Qiagen |
pRTLF-1 | rpoS-lacZ translational fusion in pMP77; Cmr | [47] |
pSB406 | contains a fusion of rhlRI'::luxCDABE in pUC18; Ampr | [48] |
pSB536 | ahyR + ahyI::luxCDABE; ColE1 origin; Ampr | [49] |
pRK2013 | Tra+ Mob+ ColE1 replicon; Kmr | [50] |
pRPO220 | pMP220 containing promoter rpoS; Tcr | [51] |
pRSAL220 | pMP220 containing promoter rsaL; Tcr | [47] |
pRHLI | pMP220 containing promoter rhlI; Tcr | This study |
pLASI | pMP220 containing promoter lasI; Tcr | This study |
pQERHLR | pQE30 containing rhlR; Ampr | This study |
pRMTA | pBBR3 containing rmtA; Tcr | This study |
pRMTC | pBBR3 containing rmtC; Tcr | This study |
Primers | ||
RhlR-fw | GGGGTACCAGGAATGACGGAGGCTTTTTG | This study |
RhlR-rv | GAAGCTTGATGAGGCCCAGCGCCGCGG | This study |
rhlI-fw | CCGAATTCCACCACAAGAACATCC | This study |
rhlI-rv | ATGGTACCAGCGATTCAGAGAGCAA | This study |
lasI-fw | GGAATTCGGGCTGTGTTCTCTCGTGTG | This study |
las-rv | CTCTAGAGAACTCTTCGCGCCGACCAA | This study |
RmtA-fw | GGGTACCAATAATTTTGTTTAACTTTA | [52] |
RmtA-rv | CCCTCGAGTCACTTATTCC | This study |
RmtC-fw | ATCTGCAGAATAATTTTGTTTAACTTTA | This study |
RmtC-rv | ATACTAGTTTACAATCTCGATACGAT | This study |