Figure 3

Pyocyanin production in PUPa3 in early stationary growth phase. All strains were grown in LB media. Presence of SIC of tobramycin in wild type significantly decreases levels of pyocyanin production (P < 0.05). Values shown in graph are means from three independent biological experiments, normalized to culture density (OD₆₀₀). WT, is strain PUPa3; Tob, is strain PUPa3 grown in the presence of SIC of tobramycin (0.05 μg/mL); Tob C4HSL, is strain PUPa3 grown in the presence of SIC of tobramycin (0.05 μg/mL) and 2 μM of C4-HSL; rhlI, is the rhlI knock-out mutant of strain PUPa3; rhlI C4HSL, is the rhlI knock-out mutant of strain PUPa3 grown in the presence of exogenously added 2 μM of C4-HSL.