CD45RA expression on CD8
HIV-specific T cells. After overnight stimulation with HIV peptide pools, PBMC were stained with fluorescent antibodies against CD8, CD45RA and IFN-γ. (a) Lymphoid CD8+ cells were gated for analysis of IFN-γ production and CD45RA expression. (b) Overnight incubation with medium alone. (c, d) Two representative samples are shown (50 and 116) with high and low fractions respectively, of CD45RA+ HIV Pol-specific CD8+ T cells producing IFN-γ following stimulation.