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Table 4 Comparison of real-time PCR detection to blood culture with known inoculants of S. Typhi into blood samples

From: The sensitivity of real-time PCR amplification targeting invasive Salmonellaserovars in biological specimens

 

Experimental condition and detection method

 

No antimicrobial

Gentamicin

Ciprofloxacin

Dilution factor

cfu/ml

Ct value

Blood culture

cfu/ml

Ct value

Blood culture

cfu/ml

Ct value

Blood culture

10-4

2.5 × 104

30.18

+

1 × 104

30.44

+

3 × 103

30.31

+

10-5

2.5 × 103

33.53

+

1 × 103

33.45

+

3 × 102

33.44

+

10-6

2.5 × 102

37.77

+

1 × 102

37.07

+

3 × 101

36.95

+

10-7

2.5 × 101

-

+

1 × 101

-

+

3 × 100

-

+

10-8

<2.5 × 101

-

+

<1 × 101

-

+

<3 × 103

-

-