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Table 3 Detection limit and Ct value comparison of PCR amplification on nucleic acid extractions from inoculated blood, inoculated PBS and purified plasmid DNA

From: The sensitivity of real-time PCR amplification targeting invasive Salmonellaserovars in biological specimens

 

Amplification targets and Ctvalue

 

S. Typhi

S. Paratyphi A

Equivalent cfu/ml

PBS

Blood

Plasmid

PBS

Blood

Plasmid

1 × 103

Ct value

21.27

24.78

27.77

31.16

-

1 × 104

Ct value

21.42

24.66

28.28

31.84

-

1 × 104

CV (%)

0.41

0.7

0.67

0.86

1.34

1 × 106

CV (%)

1.34

1.34

0.86

1.25

1.83