Amplification target | Result | Target copies | ||||
---|---|---|---|---|---|---|
 |  | 5 × 104 | 5 × 103 | 5 × 102 | 5 × 101 | 5 × 100 |
S. Typhi without internal control | Ct value* | 22.76 | 25.61 | 27.92 | 31.22 | - |
S. Typhi with internal control | Ct value | 22.07 | 25.56 | 27.76 | 31.81 | - |
Intra-assay variation†| CV (%) | 0.45 | 0.25 | 0.41 | 0.67 | 1.01 |
Inter-assay variation‡ | CV (%) | 1.86 | 1 | 1.97 | 3.39 | 1.19 |
S. Paratyphi A without internal control | Ct value | 21.27 | 24.78 | 27.77 | 31.16 | - |
S. Paratyphi A with internal control | Ct value | 21.42 | 24.66 | 28.28 | 31.84 | - |
Intra-assay variation | CV (%) | 0.41 | 0.7 | 0.67 | 0.86 | 1.34 |
Inter-assay variation | CV (%) | 1.34 | 1.34 | 0.86 | 1.25 | 1.83 |