Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR

Table 1 Used primers for the MTCD-MPCR and additional PCRs and sequencings.

Target locus	Primer name	Primer sequence	Location^a	Size (bp)	Ref. No.
MTCD-MPCR
cfp32	Rv0577F	5' ATGCCCAAGAGAAGCGAATACAGGCAA	671166-192	786	[3]
	Rv0577R	5' CTATTGCTGCGGTGCGGGCTTCAA	671951-928
RD9	Rv2073cF	5' TCGCCGCTGCCAGATGAGTC	2330579-598	600	[3]
	Rv2073cR	5' TTTGGGAGCCGCCGGTGGTGATGA	2331173-150
RD12	Rv3120F	5' GTCGGCGATAGACCATGAGTCCGTCTCCAT	3485558-587	404	[3]
	Rv3120R (390-369)	5' GCGAAAAGTGGGCGGATGCCAG	3485961-940
Additional PCRs or sequencings
cfp32	3'cfp32F	5' CGAATCATTGGCACGTCTACTTTG	671770-793	372	[2]
	3'cfp32R	5' GTGGCACCGGCGGCACCGCACACCT	672141-117
RD12	Rv3120-F (90-110)	5' GGTATTTGCGCCCATATCCTG	3485661-681	411	this study
	Rv3120-R (500-481)	5' CCTGGCTTCAAGCACCATTC	3486071-052
rpoB	rpoB-F^b	5' CAGGACGTGGAGGCGATCAC	761007-026	250	[18]
	rpoB-R	5' CAGGGGTTTCGATCGGGCAC	761256-237
rpoB ^c	rpoB-S-F^b	5' GCGTACGGTCGGCGAGCTGATCC	760922-944	418	this study
	rpoB-S-R	5' GCGGTACGGCGTTTCGATGAACC	761339-317
rrs	Bact-rrs-F^b	5' AGAGTTTGATCCTGGCTCAG	1471856-875	1496	this study
	Bact-rrs-R	5' TACGGCTACCTTGTTACGAC	1473351-332
rrs ^c	rrs-S-F^b	5' ATACCTTTGGCTCCCTTTTCC	1471809-829	1607	this study
	rrs-S-R	5' CCCACCAGTTGGGGCGTTTTC	1473415-395
hsp65	hsp65F^b	5' ACCAACGATGGTGTGTCCAT	528752-771	441	[2]
	hsp65R	5' CTTGTCGAACCGCATACCCT	529192-173
gyrB	gyrBF^b	5' ACATCAACCGCACCAAGAACGC	6027-048	483	this study
	gyrBR	5' GTGCCTTACGTGCCGCGATACG	6509-488

^aLocation on the M. tuberculosis H37Rv genome (accession no. NC_000962.2).
^bThese primers were used for PCR and sequencing.
^cThose primer pairs were used for the samples that showed non-MTC gene sequences.

ISSN: 1471-2334