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Table 4 Comparison of IF and PCR with MT-PCR Influenza profile 3.

From: Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness

  previous result MT-PCR resulta  
IF CIDM (total) 276   
INF-A (unspecified) 38 35 (H3 n = 28)
INF-B 1 1  
RSV 15 15  
hPIV-3 3 3  
total +ve 56 73 (53 +INF-A n = 14, INF-B n = 1, RSV n = 5)
-ve 220 b203  
PCR IMVS (total) 176   
INF-A (unspecified) 41 41 (H3 n = 27; H1 n = 9)
INF-B 13 13  
RSV 22 22  
hPIV-3 11 11  
total +ve 87 88 (+INF-A n = 1)c
-ve 89 85  
IF or PCR (total) 452   
all INF-A 79 90 (3 IF +ves for INF-A not detected)
all INF-B 14 14  
all RSV 37 37  
all hPIV-3 14 14  
all positive results 144 156 (+INF-A n = 15)
all negative results 308 b296  
  1. aprofile 3 includes assays for INF-A, INF-B, H1, H3, RSV, RV and hPIV3 but not H5; bnot including additional hPIV-3 (n = 2) or RSV (n = 10) detected by MT-PCR in samples which had not been tested for these pathogens; cnot including RSV/INF-A and RV/RSV co-infections