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Table 4 Comparison of IF and PCR with MT-PCR Influenza profile 3.

From: Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness

 

previous result

MT-PCR resulta

 

IF CIDM (total)

276

  

INF-A (unspecified)

38

35

(H3 n = 28)

INF-B

1

1

 

RSV

15

15

 

hPIV-3

3

3

 

total +ve

56

73

(53 +INF-A n = 14, INF-B n = 1, RSV n = 5)

-ve

220

b203

 

PCR IMVS (total)

176

  

INF-A (unspecified)

41

41

(H3 n = 27; H1 n = 9)

INF-B

13

13

 

RSV

22

22

 

hPIV-3

11

11

 

total +ve

87

88

(+INF-A n = 1)c

-ve

89

85

 

IF or PCR (total)

452

  

all INF-A

79

90

(3 IF +ves for INF-A not detected)

all INF-B

14

14

 

all RSV

37

37

 

all hPIV-3

14

14

 

all positive results

144

156

(+INF-A n = 15)

all negative results

308

b296

 
  1. aprofile 3 includes assays for INF-A, INF-B, H1, H3, RSV, RV and hPIV3 but not H5; bnot including additional hPIV-3 (n = 2) or RSV (n = 10) detected by MT-PCR in samples which had not been tested for these pathogens; cnot including RSV/INF-A and RV/RSV co-infections