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Table 2 Specific detection of haemagglutinin subtypes.

From: Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness

specimen H1 H3 H5 INF-A result
H1N1 (A/New Caledonia/20/99-like)
Cells 10-1 a63904 - - 67191 H1
Cells 10-2 4856 - - 8362 H1
Cells 10-3 2492 - - 3023 H1
Cells 10-4 188 - - 231 H1
H3N2 (A/Fijian/114/2002-like)
Cells 10-1 66 33064 - 4734 H3
Cells 10-2 - 6456 - 931 H3
Cells 10-3 - 1570 - 142 H3
Cells 10-4 - 295 - 15 H3
H5N1 (HN 3028 Vietnam; clade 1)
S/N 10-2 - - 97528 7 H5
S/N 10-3 1 - 865 3 H5
S/N 10-4 27 - 8490 25 H5
S/N 10-5 - - - 12 (INF-A)
Cells 10-1 (104/μl) 2 - 133177 1 H5
Cells 10-2 (103/μl) 5 - 116490 2 H5
Cells 10-3 (102/μl) 2 - 49011 19 H5
Cells 10-4 (101/μl) 38 - 11836 128 H5
H5N1 (Indo 05 Indonesia; clade 2)
S/N 10-1 - - 2489082 - H5
S/N 10-2 30 - 167289 6 H5
S/N 10-3 4 - 143625 6 H5
S/N 10-4 - - 156819 5 H5
Cells 10-1 (104/μl)b - - 522949 - H5
Cells 10-2 (103/μl) - - 64783 6 H5
Cells 10-3 (102/μl) 37 - 194809 1431 H5
Cells 10-4 (101/μl) 72 - 16788 9 H5
Cells 10-5 (100/μl) 19   249234 7055 H5
Cells 10-6 (10-1/μl) 9 - - 234 (INF-A)
  1. a values obtained after normalisation against internal control;
  2. b copies of virus per μl in each original sample [12]