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Table 2 Specific detection of haemagglutinin subtypes.

From: Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness

specimen

H1

H3

H5

INF-A

result

H1N1 (A/New Caledonia/20/99-like)

Cells 10-1

a63904

-

-

67191

H1

Cells 10-2

4856

-

-

8362

H1

Cells 10-3

2492

-

-

3023

H1

Cells 10-4

188

-

-

231

H1

H3N2 (A/Fijian/114/2002-like)

Cells 10-1

66

33064

-

4734

H3

Cells 10-2

-

6456

-

931

H3

Cells 10-3

-

1570

-

142

H3

Cells 10-4

-

295

-

15

H3

H5N1 (HN 3028 Vietnam; clade 1)

S/N 10-2

-

-

97528

7

H5

S/N 10-3

1

-

865

3

H5

S/N 10-4

27

-

8490

25

H5

S/N 10-5

-

-

-

12

(INF-A)

Cells 10-1 (104/μl)

2

-

133177

1

H5

Cells 10-2 (103/μl)

5

-

116490

2

H5

Cells 10-3 (102/μl)

2

-

49011

19

H5

Cells 10-4 (101/μl)

38

-

11836

128

H5

H5N1 (Indo 05 Indonesia; clade 2)

S/N 10-1

-

-

2489082

-

H5

S/N 10-2

30

-

167289

6

H5

S/N 10-3

4

-

143625

6

H5

S/N 10-4

-

-

156819

5

H5

Cells 10-1 (104/μl)b

-

-

522949

-

H5

Cells 10-2 (103/μl)

-

-

64783

6

H5

Cells 10-3 (102/μl)

37

-

194809

1431

H5

Cells 10-4 (101/μl)

72

-

16788

9

H5

Cells 10-5 (100/μl)

19

 

249234

7055

H5

Cells 10-6 (10-1/μl)

9

-

-

234

(INF-A)

  1. a values obtained after normalisation against internal control;
  2. b copies of virus per μl in each original sample [12]