Figure 6
Binding of sCD4 complexes with CAP treated and control gp120, respectively, to HIV-1 coreceptor expressing cells. CAP treated or control gp120 IIIB or MN (prepared as described for Fig. 5) were mixed with biotinylated sCD4 and added to 106 MT-2 cells (A) or PBL (B and C). The cells were washed with PBS, treated with phycoerythrin (PE)-labeled streptavidin, fixed in 1% formaldehyde and submitted to flow cytometry analysis.