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Figure 2 | BMC Infectious Diseases

Figure 2

From: Cellulose acetate phthalate, a common pharmaceutical excipient, inactivates HIV-1 and blocks the coreceptor binding site on the virus envelope glycoprotein gp120

Figure 2

Evidence for CAP binding to HIV-1 virus particles. A) Virus binding to CAP coated wells. B) Binding of CAP treated and untreated HIV-1 IIIB and BaL, respectively, to wells coated with antibodies against phthalate [reference 17]. CAP was added to a final concentration of 5 mg/ml to 50 μl of suspensions of purified HIV-1 IIIB (6.8 × 109 virus particles per ml) and HIV-1 BaL (1.8 × 1010 virus particles per ml), respectively, in 0.1 M sodium acetate pH 7.0. CAP was not added to control virus preparations. After 5 min at 37°C, HIV-1 was separated from unbound CAP as described for Fig. 1. The pellets containing HIV-1 were resuspended in 50 μl PBS containing 100 μg/ml BSA. 200 μl of 5-fold diluted virus supensions containing equal amounts of virus particles for both CAP treated and control virus (as determined by quantitation of p24 antigen), were added to wells coated as indicated above and to control wells. Bound virus was quantitated by ELISA for p24 antigen.

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