Figure 1

Inactivation of HIV-1 by CAP. Cellulose acetate phthalate (CAP) (final concentrations between 10 and 0.078 mg/ml) was added to HIV-1 IIIB containing tissue culture medium and to HIV-1 BaL, respectively. After incubation for 5 min at 37°C, the mixtures were cooled on ice and a solution of polyethylene glycol 6000 (PEG) [Reference 53] was added to a final concentration of 3% to separate HIV-1 from CAP (which does not precipitate in 3% PEG). After 90 min at 4°C, the mixtures were centrifuged at 10,000 rpm, the supernatant fluids removed and the pellets washed twice with 3% PEG in PBS containing 10 mg/ml BSA. The final pellets were resuspended in tissue culture medium and titered for infectivity. The percentage of residual infectivity is shown in a probability scale.