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Table 3 PCR Cycling Conditions: HLA DRB gene

From: Non-detection of Chlamydia species in carotid atheroma using generic primers by nested PCR in a population with a high prevalence of Chlamydia pneumoniae antibody

Cycle Conditions

First Round

PrePCRdenaturation

96°C – 5 min

Denaturation

96°C – 1 min

Annealing

55 °C – 1 min

Extension

72°C – 1 min

Number of Cycles

40

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BMC Infectious Diseases

ISSN: 1471-2334

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