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Table 2 PCR Cycling Conditions: Chlamydia genus specific

From: Non-detection of Chlamydia species in carotid atheroma using generic primers by nested PCR in a population with a high prevalence of Chlamydia pneumoniae antibody

Cycle Conditions

First Round

Second Round

PrePCRdenaturation

94°C – 3 min

94°C – 2 min

Denaturation

94°C – 10 s

94°C – 10 s

Annealing

45°C – 10 s

45°C – 10 s

Extension

72°C – 30 s

72°C – 30 s

Number of Cycles

35

35

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BMC Infectious Diseases

ISSN: 1471-2334

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