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Table 1 PCR Cycling Conditions

From: Clinical utility of a nested nucleic acid amplification format in comparison to viral culture for the diagnosis of mucosal herpes simplex infection in a genitourinary medicine setting

Cycle Conditions First Round1 Second Round1
Denaturation 94°C – 10 s 94°C – 10 s
Annealing 58°C – 10 s 67°C – 10 s
Extension 72°C – 30 s 72°C – 30 s
Number of Cycles 35 25
  1. 1The thermal cycler was held at 94°C for 3 min before cycling began to facilitate the transfer of specimens, held on ice, for a hot start procedure.