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Table 1 PCR Cycling Conditions

From: Clinical utility of a nested nucleic acid amplification format in comparison to viral culture for the diagnosis of mucosal herpes simplex infection in a genitourinary medicine setting

Cycle Conditions

First Round1

Second Round1

Denaturation

94°C – 10 s

94°C – 10 s

Annealing

58°C – 10 s

67°C – 10 s

Extension

72°C – 30 s

72°C – 30 s

Number of Cycles

35

25

  1. 1The thermal cycler was held at 94°C for 3 min before cycling began to facilitate the transfer of specimens, held on ice, for a hot start procedure.
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BMC Infectious Diseases

ISSN: 1471-2334

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