Role of systemic inflammation scores for prediction of clinical outcomes in patients treated with atazanavir not boosted by ritonavir in the Italian MASTER cohort
© The Author(s). 2017
Received: 23 December 2016
Accepted: 11 March 2017
Published: 15 March 2017
Atazanavir (ATV) not boosted by ritonavir (uATV) has been frequently used in the past for switching combination antiretroviral therapy (cART). However, the clinical outcomes and predictors of such strategy are unknown.
An observational study was carried out on the Italian MASTER, selecting HIV infected patients on cART switching to an uATV-containing regimen. Baseline was set as the last visit before uATV initiation. In the primary analysis, a composite clinical end-point was defined as the first occurring of any condition among: liver, cardiovascular, kidney, diabetes, non AIDS related cancer or death events. Incidence of AIDS events and incidence of composite clinical end-point were estimated. Kaplan-Meier and multivariable Cox regression analysis were used to assess predictors of the composite clinical end-point.
436 patients were observed. The majority of patients were males (61.5%) and Italians (85.3%), mean age was 42.7 years (IQR: 37.7–42), the most frequent route of transmission was heterosexual intercourse (47%), followed by injection drug use (25%) and homosexual contact (24%); the rate of HCV-Ab positivity was 16.3%. Patients were observed for a median time of 882 days (IQR: 252-1,769) under uATV. We recorded 93 clinical events (3 cardiovascular events, 20 kidney diseases, 33 liver diseases, 9 non AIDS related cancers, 21 diabetes, 7 AIDS events), and 19 deaths, accounting for an incidence of 3.7 (composite) events per 100 PYFU. At multivariable analysis, factors associated with the composite clinical end-point were intravenous drug use as risk factor for HIV acquisition vs. heterosexual intercourses [HR: 2.608, 95% CI 1.31–5.19, p = 0.0063], HIV RNA per Log10 copies/ml higher [HR: 1.612, 95% CI 1.278–2.034, p < 0.0001], number of switches in the nucleoside/nucleotide (NRTI) backbone of cART (performed to compose the uATV regimen under study or occurred in the past) per each more [HR: 1.085, 95% CI 1.025–1.15, p = 0.0051], Fib-4 score per unit higher [HR: 1.03, 95% CI 1.018–1.043, p < 0.0001] and Neutrophil/lymphocytes ratio (NLR inflammation score) per Log10 higher [HR: 1.319, 95% CI 1.047–1.662, p = 0.0188].
Intravenous drug users with high HIV RNA, high Fib-4 levels and more heavily exposed to antiretroviral drugs appeared to be more at risk of clinical events. Interestingly, high levels of inflammation measured through NLR, were also associated with clinical events. So, these patients should be monitored more strictly.
KeywordsHIV Atazanavir Ritonavir Clinical events Systemic inflammation scores
Atazanavir (ATV) is a protease inhibitor (PI) for the treatment of HIV infection, prescribed with ritonavir (atazanavir/ritonavir, ATV/r) in combination antiretroviral therapy (cART) [1–4]. Differently from other PIs, ATV not boosted by ritonavir (unboosted ATV, uATV) has been rather frequently used in the past as an off-label switching option [5, 6], for toxicity reasons [7, 8]. Previous studies indicated safety and effectiveness of uATV prescription in selected cART experienced patients, with undetectable HIV RNA and without drug resistances . This is why regimens including uATV have been experimented with and were used in clinical practice , and subsequently uATV prescription has been formally approved in the meantime by European Medicine Agency (EMA) and by Italian National Health authorities in 2014. At the same time, national guidelines recommended caution because of the lower genetic barrier of regimens including uATV .
Clinical outcome of patients receiving ATV (and particularly of those received uATV) is still not completely clear. In a previous study of the Italian MASTER cohort , patients treated with ATV/r more frequently achieved a composite outcome of success (HIV RNA <500 copies/mL, no AIDS events, CD4 + T cell count >500 cell/mm3) than patients prescribed other PIs  and reported a lower probability of switching the regimen than patients treated with other PIs .
However, there are some unknowns about cardiovascular risk and renal and liver profile of patients treated with uATV. Firstly, a moderate increase of lipid values (total cholesterol, HDL and LDL fractions, triglycerides) has been reported in patients prescribed ATV/r [13, 14]. Similarly, the expected increase in cardiovascular risk  was not confirmed by previous studies [16–18]. A recent study of the Italian MASTER cohort reported that the increased total cholesterol was balanced by the increased HDL fraction in patients prescribed ATV/r or uATV  and other studies observed a less impact on lipid profile in patients prescribed uATV .
Some degrees of acute and chronic interstitial nephritis, crystal deposition and nephrolithiasis were described when ATV/r was co-administered with other potentially nephrotoxic drugs, as tenofovir (TDF) [21–23]. However, an improvement of filtration rate in patients prescribed ATV/r or uATV was reported, either after switching to a regimen without TDF  or in patients prescribed ATV/r as a part of simplification regimens [13, 14]. Therefore, other factors than use of ATV may be involved in renal failure development.
Liver toxicity is a well-known side effect of ATV/r, so prescription of uATV is often adopted in case of severe hyperbilirubinaemia and cholelitiasis . However, effects of uATV prescription on liver fibrosis progression are not yet known.
Further, an increased risk of clinical events was associated with systemic inflammation levels in HIV infected patients. As recently described, neutrophil/lymphocyte ratio (NLR) measured at baseline and during follow up was independently associated with incidence of cardiovascular events , and both NLR and platelet/lymphocyte ratio (PLR) have been found to be associated with risk of death in patients with solid cancer, in general population and HIV positive subjects .
Lastly, among proposed serum non-invasive biomarkers of liver fibrosis, the Fib-4 score has been validated both in HIV/HCV co-infected and in HIV mono-infected patients and it was a reliable driver of both liver fibrosis progression and clinical events [26, 27].
The goals of present study were: (i) to estimate the risk of clinical progression (including both AIDS and non AIDS related morbidities, and death) in patients prescribed uATV; (ii) to explore possible predictors of the aforementioned outcomes, including systemic inflammation scores.
Characteristics of the Italian MASTER cohort
The present study was conducted including patients enrolled in the Italian MASTER cohort (MAnagement Standardizzato di TErapia antiRetrovirale, Standardized Management of Antiretroviral Therapy), a longitudinal multicenter cohort including patients in nine referral centers throughout Italy (http://www.mastercohort.it) . Patients’ data are recorded on a common electronic clinical chart software (Health & Notes 3.5W, Healthware S.p.A., Naples, Italy) employed by all participating Centers. The electronic clinical chart is used to record data of patients at each visit and periodically revised and updated. All participant subjects signed written informed consent and each center obtained approval by its Ethics Committee.
An observational study including HIV infected patients switching to uATV was performed. All patients were 18 years old or more. Patients were included in the study if received uATV from 2000 to 2015. Subjects included in the study were all experienced to antiretroviral therapy. Baseline was defined as the last evaluation before uATV prescription. Patients were followed from baseline (date of the last visit or exam evaluation before uATV prescription) to the time of death, switching from uATV to another regimen, or to the last available visit. The observation was interrupted also when patients were lost to follow up. Patients with any renal, liver, cardiovascular events or non AIDS related cancers before or at switch to uATV were excluded from the present study. A composite end-point (the first occurring of these conditions: liver, cardiovascular, kidney, diabetes, non AIDS related cancer or death events). Renal disease was defined as estimated glomerular filtration rate (eGFR) ≤60 ml/min calculated using Chronic Kidney Disease Epidemiology (CKD-EPI) formula , an additional measurement of eGFR within 90 days was acquired to confirm renal impairment. Clinical events of patients were recorded on an electronic clinical chart and reported following a standard classification . Cardiovascular events included diagnosis of acute myocardial infarction, stroke, transient ischemic attack, angina pectoris, coronary bypass, angioplasty, chronic occlusive arterial disease, hypertension. Liver diseases included: hyperbilirubinemia (>2.5 mg/dl), liver fibrosis stage ≥ F3, diagnosis of liver cancer. Diabetes was defined as fasting glucose ≥126 mg/dl or oral anti diabetes therapy or insulin prescription. Discontinuation of uATV was defined as uATV interruption or ATV/r prescription. Causes of uATV discontinuation were categorized as: virological failure, toxicity, simplification, other. A sensitivity analysis limited to patients who reported <4 changes of therapy in the nucleoside/nucleotide (NRTI) backbone (at baseline or in the past) was conducted, in order to exclude potential confounders due to multiple cART switches and multiple virological failures.
Baseline data collected included age, gender, nationality, risk factors for HIV acquisition, HBV and/or HCV co-infections, number of AIDS events, HIV RNA, CD4+ T cell count, ATV/r exposure, cART switches occurred for the backbone. The following parameters were also collected during the follow-up if available: CD4+ T-cell count, HIV RNA, bilirubin levels, γ-glutamyl transferase (γGT) levels, alanine transaminase (ALT) and aspartate transaminase (AST) levels, total and fractioned cholesterol, triglycerides, serum glucose, serum creatinine, platelet count, albumin, blood count, C reactive protein and coagulation markers.
Liver fibrosis estimated by Fib-4 formula , eGFR, body mass index (BMI) and systemic inflammation scores (GPS-Glasgow Prognostic Score; mGPS-modified Glasgow Prognostic Score; NLR and PLR) were calculated at baseline and during the follow up using data collected at each time point. Clinical events (AIDS, diabetes, non AIDS related cancers, cardiovascular events, renal and liver diseases), discontinuations of uATV and deaths were recorded at baseline and at each time-point.
Incidence of AIDS and non-AIDS clinical events and of the composite end-point were calculated. Kaplan-Meier analysis was performed to estimate probability of being event-free during follow up. Cox regression analysis, univariate and multivariable analyses (with bi-directional stepwise selection driven by Akaike Information Criterion, AIC), were used to assess predictors of composite clinical end-point. Data collected at baseline and during the follow-up, were input in the models as time updated variables. All analyses were performed using R, the language for statistical computing (https://www.r-project.org/).
Baseline characteristics of patients
Characteristics of the population at baseline
Risk factors for HIV acquisition
CD4+ T cell count (cells/mm3)
HIV RNA (copies/mL)
CD4+ T cell count cells/mm3
Total cholesterol (mg/dl)
Serum glucose (mg/dl)
eGFR (mL/min/1.73 m2)
Number of switches in the NRTI backbone
Predictors of clinical outcomes
Univariate and multivariable analyses for composite clinical outcome
Multivariable analysis (stepwise AIC)
HR (95% CI)
HR (95% CI)
Risk factors for HIV acquisition
MSM vs. heterosexual transmission
IDU vs. heterosexual transmission
Log10 HIV RNA (copies/mL)
LDL cholesterol (mg/dl)
Serum glucose (mg/dl)
eGFR (mL/min/1.73 m2)
Co-infections with hepatitis viruses
HBsAg unknown vs. HBsAg negative
HCV Ab unknown vs. HCV Ab negative
Number of switches in the NRTI backbone
Number of AIDS events
This study evaluated long-term clinical outcomes of HIV infected patients included in the Italian MASTER cohort after switching to a simplification regimen which included uATV. Findings of the present study are original because, for the first time, we explored the possible association of both inflammation and liver fibrosis scores with clinical events. Moreover, this is the first study which evaluated long-term clinical outcomes in patients treated with uATV.
High levels of inflammation are predictors of clinical events and mortality in HIV infected patients [30, 31]. In particular, studies from our cohort demonstrated that NLR score is an independent predictor of mortality for cancer [25, 32, 33], and cardiovascular events . Moreover, it was demonstrated that may predict death in patients with liver cirrhosis . Herein we confirm that a high NLR is a predictor of clinical events when a composite clinical outcome is used in patients prescribed uATV. Conversely, we did not observe any significant associations between clinical outcome and GPS or PLR. So, probably, these scores (GPS and PLR) are predictors of progression of disease and mortality but only in specific subgroups of HIV infected patients, such those with cancer [25, 32].
The fact that, in a subset of patients with experience of <4 changes in the NRTI backbone, NLR was not significantly associated with the composite clinical outcome may indicate that this score is more reliable in those who were more heavily exposed to cART, with a longer disease history, and with a greater risk of complications. Therefore, our results suggest that NLR warrants more attention and should be measured in HIV infected patients, especially in the most fragile, even when they are treated with “metabolic friendly” regimens (such as cART including uATV).
In the present study, we found that intravenous drug use and higher γGT levels (that may be considered as a proxy for alcohol abuse ) are associated with the composite clinical outcome. Also, higher levels of serum glucose were associated with clinical events. These findings are not unexpected because it is well known that life-style factors, such as nutritional habits, alcohol abuse, intravenous drug use or smoking exert an important role for the risk of clinical events even in HIV infected patients [27, 36–39]. So, we confirm that targeted interventions to correct these modifiable risk factors should be prioritized in HIV infected patients to reduce risk of clinical events even when more “metabolic friendly” regimens are prescribed.
The Fib-4 score is a reliable marker of liver fibrosis progression and clinical events in HIV infected patients [26, 27]. Indeed, we also found a statistically significant association of higher Fib-4 scores with the composite clinical outcome. Hence, our results suggest that patients treated with uATV with higher estimated liver fibrosis stage should be considered more at risk for clinical events. This is particularly applied to patients co-infected with HCV, for whom uATV was one of the preferred regimens [7, 8], and to those with higher NLR. Overall, these results reinforce the importance of eradicating HCV as it is the main driver of liver fibrosis progression, to reduce both liver complications and non-AIDS related co-morbidities, especially if Fib-4 and NLR remain elevated.
Results of our study are affected by several limitations. First, high incidence rates of uATV discontinuations (in most cases for “administrative” reasons to reduce off-label prescriptions) may have reduced the length of observation and, therefore, the statistical power of the study may have been limited further. However, the impact of NLR was demonstrated on the risk of both clinical complications and discontinuations of uATV.
Second, our study did not include a control group. Clearly, a control group would be of value, however, we believe that comparison with other cART regimens is not recommended in this study. Indeed, the main inclusion criteria uATV prescription; extending the patients’ selection to other cART types would require an adjustment based on the cART type. The cART effect may be so large to wash out the predictors of clinical events that we are interested in for the subpopulation of uATV. Nonetheless, we recognize the importance of comparing event rates with other regimens. An option could have been to match the uATV population for baseline characteristics. But finding suitable controls is difficult (if not impossible). Indeed, patients prescribed uATV in the past had much different characteristics with respect to patients treated with alternative regimens: in clinical practice Italian physicians tended to prescribe uATV in those with a higher rate or risk of co-morbidities, confections with viral hepatitis or intolerance to ritonavir as a booster. Moreover, it is difficult to set an appropriate baseline for the control group (i.e. patients with similar characteristics should have changed one or more drugs after a comparable time period, treatment initiation, or occurrence of certain adverse event). If the control is not suitable we would have introduced several confounder-by-indication biases.
Third, albeit alternative regimens are preferred over uATV when metabolic or liver complications are of concern, our results (especially the potential impact of persisting inflammatory state estimated through NLR) may be of general interest, provoking further investigations. Since uATV may have a smaller effect on residual HIV RNA which may increase, in its turn, levels of inflammation, it is important conduct further studies in patients receiving different cART regimens.
This work showed that NLR score is associated with clinical events in patients prescribed uATV, especially if they were intravenous drug users with uncontrolled HIV RNA, higher liver fibrosis stages, high γGT and serum glucose levels, and more heavily pre-treated with cART. In conclusion, the role of NLR in predicting the risk of non AIDS related complications and mortality should be investigated in further studies, particularly in most fragile patients (either for HCV co-infection or metabolic complications) such as those who were usually treated with uATV in clinical practice.
Akaike information criterion
Acquired immune deficiency syndrome
Body mass index
Combination antiretroviral therapy
Estimated glomerular filtration rate
Fibrosis four score
Glasgow prognostic score
Hepatitis B virus surface antigen
Hepatitis B virus
Hepatitis C virus
Human immuno-deficiency virus
Intravenous drug use
- MASTER Cohort:
Standardized Management of Antiviral Therapy Cohort
Modified Glasgow prognostic score
Men have sex with men
Non-nucleoside reverse transcriptase inhibitors
Nucleoside reverse transcriptase inhibitors
Person-year of follow-up
MASTER Cohort study group is a large national project involving the major centers providing care to HIV/AIDS patients and includes the following doctors: Francesco Castelli, Eugenia Quiros-Roldan, Paola Nasta, Malattie Infettive, Università degli Studi di Brescia; Alfredo Scalzini, Filippo Castelnuovo, Malattie Infettive Spedali Civili di Brescia; Elena Raffetti, Francesco Donato, Unità di Igiene, Epidemiologia e Sanità Pubblica, Università degli Studi di Brescia; Franco Maggiolo, Malattie Infettive Ospedale Papa Giovanni XXIII, Bergamo; Gioacchino Angarano, Nicoletta Ladisa, Clinica di Malattie Infettive Policlinico di Bari; Francesco Mazzotta, Massimo Di Pietro Malattie Infettive S.M. Annunziata, Firenze; Andrea Gori, Giuseppe Lapadula, Silvia Costarelli, Malattie Infettive Ospedale San Gerardo di Monza; Laura Sighinolfi, Malattie Infettive Nuovo Polo Ospedaliero di Cona, Ferrara; Angelo Pan, Silvia Lorenzotti, Malattie Infettive Istituti Ospitalieri Cremona; Roberto Cauda, Simona Di Giambenedetto Malattie Infettive Policlinico A. Gemelli - Università Cattolica di Roma; Carlo Torti, Università Magna Grecia di Catanzaro; Mattia Prosperi, University of Florida; Nicola Mazzini, MISI Foundation.
The MASTER Cohort was funded by MISI Foundation (Fondazione Malattie Infettive e Salute Internazionale, http://www.fondazionemisi.it/).
Availability of data and materials
For ethical and legal restriction we cannot upload a minimal data set. Data are available upon request, the interested researchers could contact directly scientific secretariat of the Italian MASTER Cohort (http://www.mastercohort.it).
MCP, CT, and MP conceived the study and participated in its design and coordination; MP performed the statistical analysis; MCP, MP and CT interpreted the data and participated in drafting the manuscript; NM participated to data management and extraction from database; EF, EQR, EDF, FR, AB, NL, MDP, AG, LS and AP participated in revision of the manuscript and contributed to patients’ enrollment in the study and follow up; EF contributed to manuscript for intellectual content. All authors read and approved the final version of the manuscript.
Prof. Carlo Torti is a member of the editorial board of BMC Infectious Diseases journal. Authors declare that they have no competing interests to declare that may bias results of this work.
Consent for publication
Ethics approval and consent to participate
The study was conducted in accordance with the guidelines of the Declaration of Helsinki and the principles of Good Clinical Practice. All patients provided written informed consent to include their clinical and biological data in the MASTER database for scientific purposes. Data submitted by the participating clinics to the data center were anonymized. The study was approved by the Ethical Committees of the Spedali Civili Hospital of Brescia (Coordinating Centre) and of the following Institutions: University Hospital of Ferrara; “Papa Giovanni XXIII” Hospital, Bergamo; University of Bari; “San Gerardo” Hospital, Monza; Hospital of Cremona; “S. M. Annunziata” Hospital, Firenze; University of Sacred Heart, Rome.
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- Piliero PJ. Atazanavir: a novel HIV-1 protease inhibitor. Expert Opin Investig Drugs. 2002;11(9):1295–301.View ArticlePubMedGoogle Scholar
- Moyle G. Overcoming obstacles to the success of protease inhibitors in highly active antiretroviral therapy regimens. AIDS Patient Care STDs. 2002;16(12):585–97.View ArticlePubMedGoogle Scholar
- De Clercq E. Highlights in the development of new antiviral agents. Mini Rev Med Chem. 2002;2(2):163–75.View ArticlePubMedGoogle Scholar
- Havlir DV, O’Marro SD. Atazanavir: new option for treatment of HIV infection. Clin Infect Dis. 2004;38(11):1599–604.View ArticlePubMedGoogle Scholar
- Focà E, et al. Unboosted atazanavir for treatment of HIV infection: rationale and recommendations for use. Drugs. 2012;72(9):1161–73.View ArticlePubMedGoogle Scholar
- Giuntini R, et al. Efficacy and safety of boosted and unboosted atazanavir-containing antiretroviral regimens in real life: results from a multicentre cohort study. HIV Med. 2010;11(1):40–5.View ArticlePubMedGoogle Scholar
- Torti C, et al. Hyperbilirubinemia during atazanavir treatment in 2,404 patients in the Italian atazanavir expanded access program and MASTER Cohorts. Infection. 2009;37(3):244–9.View ArticlePubMedGoogle Scholar
- McNicholl IR. Drug Interactions Among the Antiretrovirals. Curr Infect Dis Rep. 2004;6(2):159–62.View ArticlePubMedGoogle Scholar
- Pavie J, et al. Efficacy and safety of a switch to unboosted atazanavir in combination with nucleoside analogues in HIV-1-infected patients with virological suppression under antiretroviral therapy. J Antimicrob Chemother. 2011;66(10):2372–8.View ArticlePubMedGoogle Scholar
- Ministero della Salute. Linee guida italiane sull’utilizzo dei farmaci antiretrovirali e sulla gestione diagnositico-clinica delle persone con infezione da HIV-1. 2015; Available from: www.salute.gov.it/imgs/C_17_pubblicazioni_2442_allegato.pdf. Accessed 1 Feb 2017.
- Torti C, et al. Cohort Profile: Standardized Management of Antiretroviral Therapy Cohort (MASTER Cohort). Int J Epidemiol. 2015. [Epub ahead of print].
- Postorino MC, et al. Use of efavirenz or atazanavir/ritonavir is associated with better clinical outcomes of HAART compared to other protease inhibitors: routine evidence from the Italian MASTER Cohort. Clin Microbiol Infect. 2015;21(4):386.e1-9.View ArticlePubMedGoogle Scholar
- Mondi A, et al. Efficacy and safety of treatment simplification to atazanavir/ritonavir + lamivudine in HIV-infected patients with virological suppression: 144 week follow-up of the AtLaS pilot study. J Antimicrob Chemother. 2015;70(6):1843–9.View ArticlePubMedGoogle Scholar
- Di Giambenedetto S, et al. Safety and feasibility of treatment simplification to atazanavir/ritonavir + lamivudine in HIV-infected patients on stable treatment with two nucleos(t)ide reverse transcriptase inhibitors + atazanavir/ritonavir with virological suppression (Atazanavir and Lamivudine for treatment Simplification, AtLaS pilot study). J Antimicrob Chemother. 2013;68(6):1364–72.View ArticlePubMedGoogle Scholar
- Souza SJ, et al. Lipid profile of HIV-infected patients in relation to antiretroviral therapy: a review. Rev Assoc Med Bras. 2013;59(2):186–98.View ArticlePubMedGoogle Scholar
- Young B, et al. Inflammatory biomarker changes and their correlation with Framingham cardiovascular risk and lipid changes in antiretroviral-naive HIV-infected patients treated for 144 weeks with abacavir/lamivudine/atazanavir with or without ritonavir in ARIES. AIDS Res Hum Retroviruses. 2013;29(2):350–8.PubMedPubMed CentralGoogle Scholar
- Colafigli M, et al. Cardiovascular risk score change in HIV-1-infected patients switched to an atazanavir-based combination antiretroviral regimen. HIV Med. 2008;9(3):172–9.View ArticlePubMedGoogle Scholar
- Nguyen ST, et al. Lipid-lowering efficacy and safety after switching to atazanavir-ritonavir-based highly active antiretroviral therapy in patients with human immunodeficiency virus. Pharmacotherapy. 2008;28(3):323–30.View ArticlePubMedGoogle Scholar
- Postorino MC, et al. Exploratory analysis for the evaluation of estimated glomerular filtration rate, Cholesterol and triglycerides after switching from tenofovir/Emtricitabine plus Atazanavir/Ritonavir (ATV/r) to Abacavir/Lamivudine plus ATV/r in patients with preserved renal function. Open AIDS J. 2016;10:136–43.View ArticlePubMedPubMed CentralGoogle Scholar
- Ferraris L, et al. Switching to unboosted atazanavir reduces bilirubin and triglycerides without compromising treatment efficacy in UGT1A1*28 polymorphism carriers. J Antimicrob Chemother. 2012;67(9):2236–42.View ArticlePubMedGoogle Scholar
- Calza L, et al. Incidence of renal toxicity in HIV-infected, antiretroviral-naïve patients starting tenofovir/emtricitabine associated with efavirenz, atazanavir/ritonavir, or lopinavir/ritonavir. Scand J Infect Dis. 2013;45(2):147–54.View ArticlePubMedGoogle Scholar
- Tanaka H, et al. Evaluation of renal adverse effects of combination anti-retroviral therapy including tenofovir in HIV-infected patients. J Pharm Pharm Sci. 2013;16(3):405–13.View ArticlePubMedGoogle Scholar
- Calza L. Renal toxicity associated with antiretroviral therapy. HIV Clin Trials. 2012;13(4):189–211.View ArticlePubMedGoogle Scholar
- Quiros-Roldan E, et al. Neutrophil to lymphocyte ratio and cardiovascular disease incidence in HIV-infected patients: a population-based cohort study. PLoS ONE. 2016;11(5):e0154900.View ArticlePubMedPubMed CentralGoogle Scholar
- Raffetti E, et al. The predictive role of NLR and PLR for solid non-AIDS defining cancer incidence in HIV-infected subjects: a MASTER cohort study. Infect Agent Cancer. 2015;10:34.View ArticlePubMedPubMed CentralGoogle Scholar
- Mendeni M, et al. Evaluation of liver fibrosis: concordance analysis between noninvasive scores (APRI and FIB-4) evolution and predictors in a cohort of HIV-infected patients without hepatitis C and B infection. Clin Infect Dis. 2011;52(9):1164–73.View ArticlePubMedGoogle Scholar
- Focà E, et al. Liver fibrosis progression and clinical outcomes are intertwined: role of CD4+ T-cell count and NRTI exposure from a large cohort of HIV/HCV-coinfected patients with detectable HCV-RNA: A MASTER cohort study. Medicine (Baltimore). 2016;95(29):e4091.View ArticleGoogle Scholar
- Levey AS, et al. A new equation to estimate glomerular filtration rate. Ann Intern Med. 2009;150(9):604–12.View ArticlePubMedPubMed CentralGoogle Scholar
- Sterling RK, et al. Development of a simple noninvasive index to predict significant fibrosis in patients with HIV/HCV coinfection. Hepatology. 2006;43(6):1317–25.View ArticlePubMedGoogle Scholar
- Nordell AD, et al. Severity of cardiovascular disease outcomes among patients with HIV is related to markers of inflammation and coagulation. J Am Heart Assoc. 2014;3(3):e000844.View ArticlePubMedPubMed CentralGoogle Scholar
- McComsey GA, et al. Associations of inflammatory markers with AIDS and non-AIDS clinical events after initiation of antiretroviral therapy: AIDS clinical trials group A5224s, a substudy of ACTG A5202. J Acquir Immune Defic Syndr. 2014;65(2):167–74.View ArticlePubMedPubMed CentralGoogle Scholar
- Raffetti E, et al. The prognostic role of systemic inflammatory markers on HIV-infected patients with non-Hodgkin lymphoma, a multicenter cohort study. J Transl Med. 2015;13:89.View ArticlePubMedPubMed CentralGoogle Scholar
- Raffetti E, et al. Systemic Inflammation-Based Biomarkers and Survival in HIV-Positive Subject With Solid Cancer in an Italian Multicenter Study. J Acquir Immune Defic Syndr. 2015;69(5):585–92.View ArticlePubMedGoogle Scholar
- Biyik M, et al. Blood neutrophil-to-lymphocyte ratio independently predicts survival in patients with liver cirrhosis. Eur J Gastroenterol Hepatol. 2013;25(4):435–41.View ArticlePubMedGoogle Scholar
- Tahan V, et al. Serum gamma-glutamyltranspeptidase distinguishes non-alcoholic fatty liver disease at high risk. Hepatogastroenterology. 2008;55(85):1433–8.PubMedGoogle Scholar
- Mocroft A, et al. Serious fatal and nonfatal non-AIDS-defining illnesses in Europe. J Acquir Immune Defic Syndr. 2010;55(2):262–70.View ArticlePubMedGoogle Scholar
- Quiros-Roldan E, et al. Incidence of cardiovascular events in HIV-positive patients compared to general population over the last decade: a population-based study from 2000 to 2012. AIDS Care. 2016;28(12):1551–8.View ArticlePubMedGoogle Scholar
- Hasse B, et al. Strong Impact of Smoking on Multimorbidity and Cardiovascular Risk Among Human Immunodeficiency Virus-Infected Individuals in Comparison With the General Population. Open Forum Infect Dis. 2015;2(3):ofv108.View ArticlePubMedPubMed CentralGoogle Scholar
- Saumoy M, et al. Randomized trial of a multidisciplinary lifestyle intervention in HIV-infected patients with moderate-high cardiovascular risk. Atherosclerosis. 2016;246:301–8.View ArticlePubMedGoogle Scholar