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Association of vitamin D deficiency with hepatitis B virus - related liver diseases

BMC Infectious Diseases volume 16, Article number: 507 (2016) Cite this article

Abstract

Background

As an immune modulator, vitamin D is involved in various pathophysiological mechanisms in a plethora of diseases. This study aims to correlate the vitamin D deficiency status and clinical progression of liver diseases associated with hepatitis B virus (HBV) infection in patients in Vietnam and to compare it to healthy controls.

Methods

We quantified the levels of total vitamin D [25-(OH) D2 and D3] in serum samples from 400 HBV patients (chronic hepatitis B infection [CHB], n = 165; HBV-associated liver cirrhosis [LC], n = 127; HBV-associated hepatocellular carcinoma [HCC], n = 108) and 122 unrelated healthy controls (HC). Univariate and multivariate analyses were performed in order to determine the association between vitamin D levels and distinct clinical parameters.

Results

The prevalence of vitamin D inadequacy (<30 ng/mL) was high among healthy individuals (81.7 %) as well as in HBV patients (84.3 %). Vitamin D deficiency (<20 ng/ml) or severe deficiency (<10 ng/ml) was observed more frequently among HBV patients (52 %) and subgroups (CHB, 47.8 %; LC, 54.4 %; HCC, 55.3 %) compared to the control group (32.5 %) (P < 0.001). Vitamin D levels and HBV-DNA load were strongly and inversely correlated (rho = −0.57, P < 0.0001). Multivariate regression analysis also revealed an independent association of HBV-DNA loads with low vitamin D levels (P = 0.0004). In addition, reduced vitamin D levels were associated with significant clinical progression of LC (Child-Pugh C versus Child-Pugh A, P = 0.0018; Child-Pugh C versus Child-Pugh B, P = 0.016).

Conclusions

Vitamin D deficiency was observed in the majority of HBV-infected patients and associated with adverse clinical outcomes. Our findings suggest that substitution of vitamin D may be a supportive option in the treatment of chronic liver diseases, in particular of HBV-associated disorders.

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Background

Hepatitis B virus (HBV) infection is a major health problem which may be life-threatening due to it's frequent severe complications, including fulminant acute hepatitis, liver cirrhosis (LC) and hepatocellular carcinoma (HCC) [1]. Interactions between host immune responses and HBV determine the clinical manifestations of HBV infection. Innate immune mechanisms have important roles in the clearance of HBV through production of inflammatory cytokines such as interferons (IFN-α/β and IFN-γ) [1].

Besides the classical function in regulating calcium and bone homeostasis, vitamin D (cholecalciferol, 25-(OH)D3) is also relevant in modulating both innate and adaptive immune responses [2, 3]. Deficiency of vitamin D has been shown to be involved in carcinogenesis and the course of several infectious diseases [2–4], among them distinct liver infections [5–8].

In humans, vitamin D supplementation can result in an increased activity of intrinsic IFN-α signaling [9] and supplementation of vitamin D is a supportive option in the treatment of pulmonary tuberculosis [10, 11]. Natural sources of vitamin D are mostly vitamin D3 (cholecalciferol) retrieved from nutrition and sunlight exposure. Vitamin D2 is not produced in humans, and small amounts of vitamin D2 (ergocalciferol) are derived from edible plants [12]. Both vitamin D3 and D2 are physiologically inactive. Hydroxylation occurs in the liver, forming the intermediate metabolite (calcidiol, 25-(OH)D). Calcidiol is further converted into the active form (calcitriol, 1,25-(OH)2D) in the kidney [13] and released in the circulation. Precise measurement of calcitriol is intricate due to its low serum concentrations and short half-life. Therefore, assessment of serum concentrations of both forms of 25-(OH)D3 and D2 are required for the diagnosis of vitamin D deficiency [13].

Up to 90 % of vitamin D required for physiological functions is derived from production and supply in the skin, while the remainder is retrieved from the diet [14–16]. Production of vitamin D in the skin significantly depends on sunshine exposure, geographical habitation and skin pigmentation. However, vitamin D deficiency may occur globally. In developed countries, vitamin D deficiency is very common, with almost half of the population being affected [13]. In Vietnam, a previous study has demonstrated that vitamin D deficiency among women was approximately 30 % and, thus, almost twice of that in men (16 %) [17]. The prevalence of HBV infections currently ranges from 10 to 20 % in the Vietnamese population and HBV-related liver diseases are foreseen and predicted to be a notable public health burden in the next decades [18]. The present study aimed to study the association of vitamin D deficiency status with the progression of HBV-related liver diseases, including chronic hepatitis (CHB), liver cirrhosis (LC) and hepatocellular carcinoma (HCC) in Vietnam.

Methods

Subjects and sample collection

Four hundred Vietnamese HBV-infected patients were enrolled for this cross-sectional study at the 108 Military Central Hospital, Hanoi, Vietnam, between 2012 and 2014. HBV patients were assigned to the different clinical subgroups based on clinical manifestations. Briefly, CHB patients (n = 165) were characterized based upon clinical symptoms such as fatigue, anorexia, jaundice, hepatomegaly, hard density of the liver, splenomegaly, hyperbilirubinemia, elevated levels of AST and ALT and HBsAg positivity for more than 6 months. LC patients (n = 127) were characterized as being infected with HBV and presenting clinical manifestations such as anorexia, nausea, vomiting, malaise, weight loss, abdominal distress, jaundice, edema, cutaneous arterial spider angiomas, ascites, shrunken liver, splenomegaly, hyperbilirubinemia, elevated levels of AST and ALT, prolonged serum prothrombin time, and decreased serum albumin. HCC patients (n = 108) were characterized as being infected chronically with HBV and presenting abdominal pain, an abdominal mass in the right upper quadrant, blood-tinged ascites, weight loss, anorexia, fatigue, jaundice, prolonged serum prothrombin time, hyperbilirubinemia, elevated levels of AST, ALT and serum a-fetoprotein (AFP), ultrasound showing a tumor, and histopathology indicating tumor cells. None of the patients had a history of alcohol or drug abuse. HBV-DNA loads were measured by RT-PCR using the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, California, USA) and liver function tests, including assessment of alanine transaminase (ALT) and aspartate transaminase (AST) enzyme levels, alpha fetoprotein (AFP), platelet count (PLT), total and direct bilirubin, albumin and prothrombin were performed using AU640 Chemistry Analyzer (Beckman Coulter, California, USA). Based on Child-Pugh scores, HBV-related LC patients were categorized as Child-Pugh A, −B and -C [19]. In addition, 122 healthy individuals devoid of HBsAg were randomly enrolled from the hospital’s blood bank as controls. None of these healthy individuals had a history of any chronic liver disease, nor were they immunosuppressed. All participants were confirmed negative for anti-HCV and anti-HIV antibodies by using commercial ELISA assays (Diagnostic automation/Cortez Diagnostics, Inc., Woodland Hills, California, USA).

Vitamin D measurement

Total vitamin D [25-(OH)D2 and D3] levels were measured in the serum samples from patients and controls using a commercial ELISA kit (Gentaur, Kampenhout, Belgium) according to the manufacturer’s instructions. The kit’s range of vitamin D assessment is 4.39 to 133 ng/ml with a coefficient of variation of 20 %. Based on the recommendation of the Endocrine Society (Maryland, USA; http://www.endocrine.org/) [20], a level of 30 ng/ml or above is considered as vitamin D sufficiency. We, therefore, categorized the vitamin D status as normal (≥30 ng/ml), insufficient (20–29.9 ng/ml), deficient (10–19.9 mg/ml), and severely deficient (<10 ng/ml).

Statistics

All statistical analyses were performed using R version 3.1.2 (http://www.r-project.org). Chi-square test, Kruskal-Wallis test, and Mann-Whitney-Wilcoxon test were used to compare differences between groups for qualitative or quantitative variables where appropriate. Correlations between variables were evaluated using the Spearman’s rank correlation test. We used univariate analyses, multivariate logistic regression and multivariate linear regression models to test for associations of serum 25-(OH)D3 status with clinical parameters and independent risk factors. Bonferroni correction was applied as appropriate for multiple comparisons. The level of significance was set at P < 0.05.

Results

Patients and control characteristics

The baseline characteristics of the 400 HBV-infected patients and 122 healthy controls are given in Table 1. The majority of both patients and healthy controls were males (85.3 and 67.2 %, respectively). As expected, the median age of patients increased with progression of liver disease. Healthy controls were significantly younger than patients (P < 0.001). The levels of ALT and AST were significantly higher in patients with CHB compared to those with LC and HCC (ALT: P < 0.001 and AST: P = 0.014). As also expected, albumin and prothrombin levels as well as platelet counts were significantly lower in patients with LC compared to those without LC (P < 0.01). AFP levels were significantly higher among the HCC group compared to the CHB and LC groups (P < 0.001).

Table 1 Characteristics of the study cohort
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Serum vitamin D levels in HBV infected patients and in controls

Seven individuals, each two from the HC and the CHB groups, and three from the HCC group had undetectable vitamin D levels (<4.39 ng/ml). Vitamin D levels were mostly inadequate (<30 ng/ml) in both HBV patients (84.3 %) and controls (81.7 %); the difference, however, was not significant. When vitamin D levels were stratified into the four categories of normal levels (≥30 ng/ml), insufficiency (20–29.9 ng/ml), deficiency (10–19.9 mg/ml), and severe deficiency (<10 ng/ml), deficiency and severe deficiency occurred frequently among all HBV patients (51.9 %) and in all subgroups compared to healthy controls (32.5 %) (P < 0.001) (Table 2). Vitamin D levels were 20.7 ± 9.2 ng/ml in HBV-infected patients and 23.6 ± 9.5 ng/ml in HCs. In the patient subgroups, vitamin D levels were 21.2 ± 8.9 ng/ml in CHB, 20.6 ± 10.4 ng/ml in LC, and 20 ± 8.3 ng/ml in HCC. Vitamin D levels were higher in HCs than in HBV patients (HBV patients vs. HC: P = 0.0014; LC vs. HC: P = 0.0049; HCC vs. HC: P = 0.0028) (Fig. 1). Among the HBV patients, vitamin D levels were decreased with progression of HBV-related liver diseases. However, significant differences were not observed when comparing the subgroups (CHB vs. LC, CHB vs. HCC and LC vs. HCC) (Fig. 1). Deficiency and severe vitamin D deficiency were significantly associated with advanced LC according to Child-Pugh classifications (Fig. 2a and b). Of 127 LC patients, 30.4 % patients of the Child-Pugh C group had severe deficiency, while 10 % only in the Child-Pugh A and B groups had severe deficiency (Fig. 2a). In addition, vitamin D levels were significantly lower in Child-Pugh C patients (15.1 ± 6.5 ng/ml), compared to Child-Pugh B (20.8 ± 10.6 ng/ml) and Child-Pugh A patients (23.3 ± 10.7 ng/ml) (P = 0.0018 and P = 0.016, respectively) (Fig. 2b).

Table 2 Distribution of HBV-infected patients and healthy controls with Vitamin D status
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Fig. 1
figure 1

Vitamin D levels in healthy individuals (HC), HBV patients and subgroups. Chronic hepatitis B (CHB), liver cirrhosis (LC) and hepatocellular carcinoma (HCC); P values were calculated by Mann-Whitney Wilcoxon test

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Fig. 2
figure 2

Distribution of vitamin D levels in HBV related liver cirrhosis patients. a Vitamin D levels in different stages of HBV-related liver cirrhosis according to Child-Pugh classification. b Vitamin D levels in different stages of HBV-related liver cirrhosis. Dot-plots illustrate medians with interquartile ranges. P values were calculated by Mann-Whitney-Wilcoxon test

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Factors associated with vitamin D levels and vitamin D deficiency

Of 165 CHB patients, 63 only were available for analyses of the correlation between vitamin D levels and HBV-DNA loads. Vitamin D levels and HBV-DNA were significantly and inversely correlated (rho = −0.57, P < 0.0001) (Fig. 3). Other clinical parameters were either not or weakly correlated with vitamin D serum levels. This applied to the PLT (rho = 0.05), ALT (rho = 0.16), AST (rho = 0.20), total-bilirubin (rho = 0.03), direct-bilirubin (rho = 0.04), albumin (rho = 0.002), AFP (rho = −0.02) and prothrombin levels (rho = 0.07) (data not shown). We also performed multivariate linear regression models to determine the independent factors correlated with vitamin D levels. Only HBV-DNA loads were independently and inversely correlated with vitamin D levels (P = 0.0004) (Table 3).

Fig. 3
figure 3

Correlation between Vitamin D levels with HBV-DNA loads. The correlation between vitamin D levels and HBV-DNA loads in chronic hepatitis B patients was calculated by using Spearman’s rank correlation coefficient. Spearman’s rho (rho) and P value are given

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Table 3 Factors associated with baseline vitamin D levels in HBV patients
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Next, we applied univariate analysis and multivariate logistic regression models to assess the determinants associated with vitamin D deficiency. HBV infection and HBeAg negativity were independently associated with low vitamin D levels (<30 ng/ml) in both univariate and multivariate analyses. HCC was also a risk factor for low vitamin D levels in the univariate analysis (Table 4). Other independent factors such as age, gender, and the occurrence of LC were not associated with any degree of vitamin D deficiency.

Table 4 Univariate and multivariate logistic regression analyses of factors associated with vitamin D deficiency
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Discussion

Vitamin D has not only important functions in the metabolism of calcium and bone homeostasis but also manifold effects in the fine regulation of immune responses [2, 3, 13]. There is increasing evidence of vitamin D deficiency effects on a wide spectrum of diseases, including osteoporosis, autoimmunity, asthma, infectious diseases, several forms of malignancy and even psychiatric disorders [13, 21, 22]. This study shows that insufficiency of vitamin D occurs more frequently among healthy individuals as well as HBV patients. Vitamin D levels were inversely correlated with HBV-DNA loads and were associated with more severe conditions of LC patients. These findings suggest that serum vitamin D levels contribute significantly to the clinical courses of HBV infection, including the severe consequences of LC and HCC.

A high prevalence of vitamin D deficiency (>90 %) in chronic liver disease has been reported to be associated with liver disease progression [23–25]. High prevalence of inadequate vitamin D status, as observed in both healthy populations and in patients with HBV-related liver diseases, indicate that low vitamin D levels are frequent, along with osteoporosis in populations of many geographical regions [13, 26]. Roughly, one billion people worldwide apparently are vitamin D deficient [13, 27]. Vitamin D deficiency occurs rather among the elderly population [28, 29] and is more frequent in women [13, 17]. In this study, the predominance of males in all study groups and the significant difference in age between cases and healthy individuals might influence the degree of vitamin D deficiency between cases and healthy individuals. In the multivariate analyses adjusted for age and gender, HBV infection appears to be an independent risk factor for vitamin D inadequacy that augments the risk for osteoporosis and other vitamin D deficiency-related diseases. The high prevalence of HBV infection in Vietnam (10–20 %) [18] is an important factor favoring the occurrence of osteoporosis and many other diseases related to vitamin D deficiency.

In this study, deficiency and severe vitamin D deficiency were observed more frequently in patients with HBV-related liver disease and were found significantly associated with the end-stage of liver cirrhosis (Child-Pugh C). Vitamin D is involved in inhibition of inflammation and abrogation of liver fibrosis, substantiated by the observation that vitamin D receptor knockout mice spontaneously develop hepatic fibrosis [30, 31]. Apparently, vitamin D deficiency is a cause of fibrosis progression [6, 8, 32]. The interaction between vitamin D and its receptor can modulate the immune response involved in the pathogenesis of many solid cancers [33, 34]. Our study is in line with previous studies which have indicated an association of vitamin D levels with HCC [35–38]. Vitamin D can inhibit the growth of HCC cell lines both in vitro and in vivo [39], and supplementation of vitamin D and calcium has substantially reduced the risk of cancer in a randomized clinical trial [40]. In addition, vitamin D analogs and vitamin D receptor activators such as maxacalcitol (OCT), 16-ene analogs, 19-nor analogs, LG190119 and C-20 cyclopropylcal-citriol have been developed and tested in a pre-clinical study [41–43]. Vitamin D analogs and VDR activators may in the future be promising adjuvants in HCC treatment.

The causes of vitamin D deficiency in chronic liver disease are considered multi-factorial [13, 21]. An explanation for the low vitamin D levels observed in chronic liver disease could be an impaired liver function. Hepatic injuries lead to decreased production of vitamin D carrier proteins such as vitamin D binding protein and albumin, and impaired hepatic hydroxylation of vitamin D to calcidiol or 25-(OH)D in the liver. Vitamin D levels were shown to be positively correlated with albumin levels and platelet counts and inversely correlated with ALT levels during the active phase of chronic liver disease [6, 24, 25]. Serum vitamin D levels of <10 ng/ml can be a predictive factor for low serum albumin levels and the severity of chronic liver disease [32, 44]. In the present study, however, vitamin D inadequacy was not significantly correlated with liver function parameters, possibly due to the fact that vitamin D serum levels are affected by multiple factors and most of the patients were not in the active phase during sample collection.

In line with a previous study [45], our results corroborate a strongly inverse correlation between HBV-DNA loads and vitamin D levels. We assume that vitamin D deficiency, as frequently observed in HBV infection, can fail to suppress HBV replication. A link between vitamin D deficiency and susceptibility to various infections and the development of various inflammatory diseases has been suggested [2, 3]. In addition, low vitamin D levels linked to low sustained virological response to interferon-based therapy in chronic hepatitis B and C have been demonstrated in clinical studies [8, 25]. Although vitamin D deficiency is associated with adverse clinical outcomes of HBV infection, our study has limitations. Serum vitamin D levels are affected by several factors, including seasonal variation, sunshine exposure, geographical habitation and diet. However, any information concerning the season at the time of sampling and diet of the study subjects was not available. Another limitation is that the study was a cross-sectional study and, thus, we could not determine the fluctuation of vitamin D levels over the course of HBV infection as well as the causative association of vitamin D levels and HBV-related liver diseases. Vitamin D may be an additional factor for the clinical outcomes of HBV infection. The influence of vitamin D metabolism on HBV-related liver diseases remains unclear and the role of vitamin D and its interaction with vitamin D receptor on the pathogenesis of HBV infection needs to be explored further.

Conclusions

Vitamin D deficiency was observed in the majority of HBV patients particularly in advanced liver diseases and associated with adverse clinical outcomes. Our findings allow speculating that vitamin D and its analogs might provide a potential therapeutic addendum in the treatment of chronic liver diseases.

Abbreviations

AFP:

Alpha feto protein

AST and ALT:

Aspartate and alanine amino transferase

CHB:

Chronic hepatitis B

HBV:

Hepatitis B virus

HC:

Healthy control

HCC:

Hepatocellular carcinoma

LC:

Liver cirrhosis

PLT:

Platelets

References

  1. Trepo C, Chan HL, Lok A. Hepatitis B virus infection. Lancet. 2014;384:2053–63.

    Article  CAS  PubMed  Google Scholar 

  2. Deluca HF, Cantorna MT. Vitamin D: its role and uses in immunology. FASEB J. 2001;15:2579–85.

    Article  CAS  PubMed  Google Scholar 

  3. Aranow C. Vitamin D and the immune system. J Investig Med. 2011;59:881–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Plum LA, Deluca HF. Vitamin D, disease and therapeutic opportunities. Nat Rev Drug Discov. 2010;9:941–55.

    Article  CAS  PubMed  Google Scholar 

  5. Manco M, Ciampalini P, Nobili V. Low levels of 25-hydroxyvitamin D(3) in children with biopsy-proven nonalcoholic fatty liver disease. Hepatology. 2010;51:2229.

    Article  PubMed  Google Scholar 

  6. Targher G, Bertolini L, Scala L, Cigolini M, Zenari L, Falezza G, et al. Associations between serum 25-hydroxyvitamin D3 concentrations and liver histology in patients with non-alcoholic fatty liver disease. Nutr Metab Cardiovasc Dis. 2007;17:517–24.

    Article  CAS  PubMed  Google Scholar 

  7. Barchetta I, Angelico F, Del BM, Baroni MG, Pozzilli P, Morini S, et al. Strong association between non alcoholic fatty liver disease (NAFLD) and low 25(OH) vitamin D levels in an adult population with normal serum liver enzymes. BMC Med. 2011;9:85.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Petta S, Camma C, Scazzone C, Tripodo C, Di Marco V, Bono A, et al. Low vitamin D serum level is related to severe fibrosis and low responsiveness to interferon-based therapy in genotype 1 chronic hepatitis C. Hepatology. 2010;51:1158–67.

    Article  CAS  PubMed  Google Scholar 

  9. Coussens AK, Wilkinson RJ, Hanifa Y, Nikolayevskyy V, Elkington PT, Islam K, et al. Vitamin D accelerates resolution of inflammatory responses during tuberculosis treatment. Proc Natl Acad Sci U S A. 2012;109:15449–54.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Daley P, Jagannathan V, John KR, Sarojini J, Latha A, Vieth R, et al. Adjunctive vitamin D for treatment of active tuberculosis in India: a randomised, double-blind, placebo-controlled trial. Lancet Infect Dis. 2015;15:528–34.

    Article  CAS  PubMed  Google Scholar 

  11. Martineau AR, Timms PM, Bothamley GH, Hanifa Y, Islam K, Claxton AP, et al. High-dose vitamin D(3) during intensive-phase antimicrobial treatment of pulmonary tuberculosis: a double-blind randomised controlled trial. Lancet. 2011;377:242–50.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Heaney RP, Recker RR, Grote J, Horst RL, Armas LA. Vitamin D(3) is more potent than vitamin D(2) in humans. J Clin Endocrinol Metab. 2011;96:E447–52.

    Article  CAS  PubMed  Google Scholar 

  13. Holick MF. Vitamin D deficiency. N Engl J Med. 2007;357:266–81.

    Article  CAS  PubMed  Google Scholar 

  14. Holick MF. McCollum Award Lecture, 1994: vitamin D–new horizons for the 21st century. Am J Clin Nutr. 1994;1994(60):619–30.

    Google Scholar 

  15. Holick MF. Vitamin D and bone health. J Nutr. 1996;126:1159S–64S.

    CAS  PubMed  Google Scholar 

  16. Holick MF, Chen TC. Vitamin D deficiency: a worldwide problem with health consequences. Am J Clin Nutr. 2008;87:1080S–6S.

    CAS  PubMed  Google Scholar 

  17. Nguyen HT, von SB, Nguyen TV, Dzung DN, Duc PT, Thuy VT, et al. Vitamin D deficiency in northern Vietnam: prevalence, risk factors and associations with bone mineral density. Bone. 2012;51:1029–34.

    Article  CAS  PubMed  Google Scholar 

  18. Nguyen VT. Hepatitis B infection in Vietnam: current issues and future challenges. Asia Pac J Public Health. 2012;24:361–73.

    Article  PubMed  Google Scholar 

  19. Child CG. Surgery and portal hypertension. In: The liver and portal hypertension. Philadelphia: WB Saunders; 1964. p. 50–72.

    Google Scholar 

  20. Dawson-Hughes B, Heaney RP, Holick MF, Lips P, Meunier PJ, Vieth R. Estimates of optimal vitamin D status. Osteoporos Int. 2005;16:713–6.

    Article  CAS  PubMed  Google Scholar 

  21. Rosen CJ. Clinical practice. Vitamin D insufficiency. N Engl J Med. 2011;364:248–54.

    Article  CAS  PubMed  Google Scholar 

  22. van den Berg KS, Marijnissen RM, van den Brink RH, Naarding P, Comijs HC, Oude Voshaar RC. Vitamin D deficiency, depression course and mortality: longitudinal results from the Netherlands Study on Depression in Older persons (NESDO). J Psychosom Res. 2016;83:50–6.

    Article  PubMed  Google Scholar 

  23. Arteh J, Narra S, Nair S. Prevalence of vitamin D deficiency in chronic liver disease. Dig Dis Sci. 2010;55:2624–8.

    Article  CAS  PubMed  Google Scholar 

  24. Wong GL, Chan HL, Chan HY, Tse CH, Chim AM, Lo AO, et al. Adverse effects of vitamin D deficiency on outcomes of patients with chronic hepatitis B. Clin Gastroenterol Hepatol. 2015;13:783–90.

    Article  CAS  PubMed  Google Scholar 

  25. Chan HL, Elkhashab M, Trinh H, Tak WY, Ma X, Chuang WL, et al. Association of baseline vitamin D levels with clinical parameters and treatment outcomes in chronic hepatitis B. J Hepatol. 2015;63:1086–92.

    Article  CAS  PubMed  Google Scholar 

  26. Boonen S, Bischoff-Ferrari HA, Cooper C, Lips P, Ljunggren O, Meunier PJ, et al. Addressing the musculoskeletal components of fracture risk with calcium and vitamin D: a review of the evidence. Calcif Tissue Int. 2006;78:257–70.

    Article  CAS  PubMed  Google Scholar 

  27. Holick MF, Siris ES, Binkley N, Beard MK, Khan A, Katzer JT, et al. Prevalence of Vitamin D inadequacy among postmenopausal North American women receiving osteoporosis therapy. J Clin Endocrinol Metab. 2005;90:3215–24.

    Article  CAS  PubMed  Google Scholar 

  28. Mosekilde L. Vitamin D and the elderly. Clin Endocrinol (Oxf). 2005;62:265–81.

    Article  CAS  Google Scholar 

  29. Larsen ER. Fall and fractures among elderly community residents: risk factors and prevention. Faculty of Health Sciences, University of Aarhus, Denmark; 2002

  30. Ding N, Yu RT, Subramaniam N, Sherman MH, Wilson C, Rao R, et al. A vitamin D receptor/SMAD genomic circuit gates hepatic fibrotic response. Cell. 2013;153:601–13.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Abramovitch S, Dahan-Bachar L, Sharvit E, Weisman Y, Ben TA, Brazowski E, et al. Vitamin D inhibits proliferation and profibrotic marker expression in hepatic stellate cells and decreases thioacetamide-induced liver fibrosis in rats. Gut. 2011;60:1728–37.

    Article  CAS  PubMed  Google Scholar 

  32. Fisher L, Fisher A. Vitamin D and parathyroid hormone in outpatients with noncholestatic chronic liver disease. Clin Gastroenterol Hepatol. 2007;5:513–20.

    Article  CAS  PubMed  Google Scholar 

  33. Ma Y, Zhang P, Wang F, Yang J, Liu Z, Qin H. Association between vitamin D and risk of colorectal cancer: a systematic review of prospective studies. J Clin Oncol. 2011;29:3775–82.

    Article  CAS  PubMed  Google Scholar 

  34. Lamprecht SA, Lipkin M. Cellular mechanisms of calcium and vitamin D in the inhibition of colorectal carcinogenesis. Ann N Y Acad Sci. 2001;952:73–87.

    Article  CAS  PubMed  Google Scholar 

  35. Lange CM, Miki D, Ochi H, Nischalke HD, Bojunga J, Bibert S, et al. Genetic analyses reveal a role for vitamin D insufficiency in HCV-associated hepatocellular carcinoma development. PLoS One. 2013;8:e64053.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Fingas CD, Altinbas A, Schlattjan M, Beilfuss A, Sowa JP, Sydor S, et al. Expression of apoptosis- and vitamin D pathway-related genes in hepatocellular carcinoma. Digestion. 2013;87:176–81.

    Article  CAS  PubMed  Google Scholar 

  37. Chiang KC, Yeh CN, Chen MF, Chen TC. Hepatocellular carcinoma and vitamin D: a review. J Gastroenterol Hepatol. 2011;26:1597–603.

    Article  CAS  PubMed  Google Scholar 

  38. Fedirko V, Duarte-Salles T, Bamia C, Trichopoulou A, Aleksandrova K, Trichopoulos D, et al. Prediagnostic circulating vitamin D levels and risk of hepatocellular carcinoma in European populations: a nested case-control study. Hepatology. 2014;60:1222–30.

    Article  CAS  PubMed  Google Scholar 

  39. Pourgholami MH, Akhter J, Lu Y, Morris DL. In vitro and in vivo inhibition of liver cancer cells by 1,25-dihydroxyvitamin D3. Cancer Lett. 2000;151:97–102.

    Article  CAS  PubMed  Google Scholar 

  40. Lappe JM, Travers-Gustafson D, Davies KM, Recker RR, Heaney RP. Vitamin D and calcium supplementation reduces cancer risk: results of a randomized trial. Am J Clin Nutr. 2007;85:1586–91.

    CAS  PubMed  Google Scholar 

  41. Brown AJ, Slatopolsky E. Vitamin D analogs: therapeutic applications and mechanisms for selectivity. Mol Aspects Med. 2008;29:433–52.

    Article  CAS  PubMed  Google Scholar 

  42. Fujii H, Nakai K, Yonekura Y, Kono K, Goto S, Hirata M, et al. The vitamin D receptor activator maxacalcitol provides cardioprotective effects in diabetes mellitus. Cardiovasc Drugs Ther. 2015;29:499–507.

    Article  CAS  PubMed  Google Scholar 

  43. Chiang KC, Yeh CN, Hsu JT, Yeh TS, Jan YY, Wu CT, et al. Evaluation of the potential therapeutic role of a new generation of vitamin D analog, MART-10, in human pancreatic cancer cells in vitro and in vivo. Cell Cycle. 2013;12:1316–25.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Rode A, Fourlanos S, Nicoll A. Oral vitamin D replacement is effective in chronic liver disease. Gastroenterol Clin Biol. 2010;34:618–20.

    Article  CAS  PubMed  Google Scholar 

  45. Farnik H, Bojunga J, Berger A, Allwinn R, Waidmann O, Kronenberger B, et al. Low vitamin D serum concentration is associated with high levels of hepatitis B virus replication in chronically infected patients. Hepatology. 2013;58:1270–6.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We would like to thank all the study subjects and blood donors for their participation and extend our thanks to Le Duc Anh, Nguyen Viet Ha for the help of sample collection.

Funding

We acknowledge the support from DAAD-PAGEL (57140033) for student fellowship. The funders have no role in the study design, data collection and analysis, decision to publish and/or preparation of the manuscript.

Availability of data and materials

Data and supporting materials associated with this study will be shared upon request.

Authors’ contributions

LHS, DTA, TPV and PGK designed and supervised the studies. NXH, NK, MTB, DPG and HVT conducted the experiments. LHS, NXH, MTB, DTA, NK, PQH, NTT, NLT evaluated the clinical data and provided the clinical samples. NXH, HVT, CGM, TPV and LHS analyzed the data and wrote the manuscript. All authors read and approved the manuscript.

Competing interests

All authors declare that they have no competing interests.

Consent for publication

Not applicable.

Ethics approval and consent to participate

The study was approved by the Institutional Review Board of the 108 Military Central Hospital, Hanoi, Vietnam and the Military Hospital 103 of the Vietnam Military Medical University, Hanoi, Vietnam. Informed written consents were obtained at the time of sampling from all the participants.

Author information

Author notes

    Authors and Affiliations

    1. Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany

      Nghiem Xuan Hoan, Mai Thanh Binh, Dao Phuong Giang, Hoang Van Tong, Christian G. Meyer, Peter G. Kremsner & Thirumalaisamy P. Velavan

    2. Vietnamese-German Center for Medical Research (VG-CARE), Hanoi, Vietnam

      Nghiem Xuan Hoan, Mai Thanh Binh, Dao Phuong Giang, Hoang Van Tong, Ngo Tat Trung, Nguyen Linh Toan, Christian G. Meyer, Peter G. Kremsner, Thirumalaisamy P. Velavan & Le Huu Song

    3. Department of Infectious Diseases, Duc Giang Hospital, Hanoi, Vietnam

      Nguyen Khuyen

    4. Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam

      Phan Quoc Hoan & Ngo Tat Trung

    5. Department of Infectious Diseases, Vietnam Military Medical University, Hanoi, Vietnam

      Do Tuan Anh

    6. Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam

      Nguyen Linh Toan

    7. Fondation Congolaise pour la Recherche Medicale, Brazzaville, Republic of Congo

      Thirumalaisamy P. Velavan

    8. Institute of Clinical Infectious Diseases, 108 Military Central Hospital, Tran Hung Dao Street N1, Hai Ba Trung District, Hanoi, Vietnam

      Le Huu Song

    Authors
    1. Nghiem Xuan Hoan
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    2. Nguyen Khuyen
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    3. Mai Thanh Binh
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    4. Dao Phuong Giang
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    5. Hoang Van Tong
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    6. Phan Quoc Hoan
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    7. Ngo Tat Trung
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    8. Do Tuan Anh
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    9. Nguyen Linh Toan
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    10. Christian G. Meyer
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    11. Peter G. Kremsner
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    12. Thirumalaisamy P. Velavan
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    13. Le Huu Song
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    Corresponding author

    Correspondence to Le Huu Song.

    Additional information

    Thirumalaisamy P. Velavan and Le Huu Song shared senior authorship

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    Hoan, N.X., Khuyen, N., Binh, M.T. et al. Association of vitamin D deficiency with hepatitis B virus - related liver diseases. BMC Infect Dis 16, 507 (2016). https://doi.org/10.1186/s12879-016-1836-0

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    • DOI: https://doi.org/10.1186/s12879-016-1836-0

    Keywords

    BMC Infectious Diseases

    ISSN: 1471-2334

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