Drug-resistance in Streptococcus pneumoniae isolates among Spanish middle aged and older adults with community-acquired pneumonia
© Vila-Corcoles et al; licensee BioMed Central Ltd. 2009
Received: 06 November 2008
Accepted: 25 March 2009
Published: 25 March 2009
Pneumococcal diseases remain a major cause of morbidity and mortality worldwide. Updated data on drug-resistance from different populations may be important to recognize changes in disease patterns. This study assessed current levels of penicilin resistance among Streptococcus Pneumoniae causing pneumonia in Spanish middle age and older adults.
Antimicrobial susceptibility was tested for 104 consecutive isolates of Streptococcus pneumoniae recovered from patients 50 years or older with radiographically confirmed pneumonia in the region of Tarragona (Spain) between 2002 and 2007. According to the minimum inhibitory concentration of tested antimicrobials (penicillin, erythromycin, cefotaxime and levofloxacin) strains were classified as susceptible or resistant. Antimicrobial resistance was determined for early cases (2002–2004) and contemporary cases (2005–2007).
Twenty-seven (25.9%) were penicillin-resistant strains (19 strains with intermediate resistance and 8 strains with high resistance). Penicillin-resistance was higher in 2002–2004 than in 2005–2007 (39.5% vs 18.2%, p = 0.017).
Of 27 penicillin-resistant strains, 10 (37%) were resistant to erythromycin, 8 (29.6%) to cefotaxime, 2 (7.4%) to levofloxacin, and 4 (14.8%) were identified as multidrug resistant. Case-fatality rate was higher among those patients who had an infection caused by any penicillin susceptible strain (16.9%) than in those with infections due to penicillin-resistant strains.
Resistance to penicillin among Streptococcus pneumoniae remains high, but such resistance does not result in increased mortality in patients with pneumococcal pneumonia.
Streptococcus pneumoniae is the most frequent isolate from clinical samples of respiratory tract infection, including acute exacerbations of chronic bronchitis and community-acquired pneumonia [1–3]. The emergence of multiple drug-resistance has complicated the empirical treatment of pneumococcal infections. Several surveillance programs that span numerous countries indicate that the proportion of drug-resistant Streptococcus pneumoniae isolates continues to increase worldwide. However, in Spain, where there was a great increase of penicillin-resistance in 1980s, stabilization in 1990s and a decrease in penicillin-resistant isolates in the last few years has been reported [5–7].
In elderly people, the population group with highest rates of pneumococcal infection, the penicillin intermediate-susceptibility strains has not been associated with increased mortality or unsuccessful treatments, so these strains can be treated with β-lactams. If we consider penicillin-resistant pneumococci, data reported on mortality has not been uniform[4, 8, 9].
Updated data about drug-resistance in Streptococcus pneumoniae isolates from different populations may be important in recognizing changes in disease patterns. The present study describes current levels of drug resistance among Streptococcus pneumoniae causing community-acquired pneumonia in Spanish middle aged and older patients, and evaluates clinical factors associated with increasing drug resistance or mortality.
Hospital case-series that included 104 consecutively recruited isolates of Streptococcus pneumoniae recovered from patients 50 years or older with a radiographycally confirmed community-acquired pneumonia in the region of Tarragona (a mixed residential industrial urban area in the Northeast Mediterranean coast of Spain) with Antimicrobial susceptibility to penicillin tested between January 2002 and April 2007.
The isolates of S. pneumoniae were identified from blood and sputum samples in the microbiology laboratory of two reference hospitals in the study area (Joan XXIII and Sant Pau i Santa Tecla) by standard methods. Pneumococcal serotyping was carried out by the Quellung reaction using the Statens Serum Institute (Copenhagen, Denmark) typing sera. Penicillin susceptibility was determined by the Kirby-Bauer disk diffusion method with a disk containing 1 μg oxacillin (BD BBL, Sparks, MD USA). Isolates were defined as resistant when the growth inhibition diameter was 19 mm or less. Minimal inhibitory concentration (MIC) for penicillin and cefotaxime was determined by E-test method (AB Biodisk, Solna, Sweden) following the manufacturer's instructions. Penicillin-susceptible was considered when MIC ≤ 0.06 μg/mL, penicillin intermediate-resistant when MIC 0.12 – 1 μg/mL and high resistance when MIC ≥ 2 μg/mL. Cefotaxime susceptibility was considered when MIC ≤ 1 μg/mL, cefotaxime intermediate when MIC 2 μg/mL and cefotaxime resistant when MIC ≥ 4 μg/mL. Erythromycin and levofloxacin susceptibility was determined by the Kirby-Bauer disk diffusion method with a disk containing 15 μg erythromycin and 5 μg levofloxacin respectively (BD BBL, Sparks, MD USA). Erythromycin susceptibility was considered when the growth inhibition diameter was ≥ 21 mm, erythromycin intermediate between 16 and 20 mm, and erythromycin resistant ≤ 15 mm. Levofloxacin susceptibility was considered when the growth inhibition diameter was ≥ 17 mm, levofloxacin intermediate between 14 and 16 mm, and levofloxacin resistant ≤ 13 mm, according to the guidelines established by the National Committee for Clinical and Laboratory Standards (formerly NCCLS). Multidrug resistance was defined as intermediate resistance or resistance to penicillin plus intermediate resistance or resistance to ≥ 2 antimicrobial agents.
Cases were validated by checking clinical records and, according to the presence of underlying medical conditions, all patients were grouped into 3 risk strata on the basis of the degree of immunocompromise and risk for pneumococcal disease. High-risk level included persons with conditions associated with possible immunocompromise: immunodeficiency (including AIDS), asplenia, cancer (solid organ or haematological neoplasia), chronic nephropathy (nephrotic syndrome, renal failure, dialysis or transplantation), and long-term corticosteroid therapy (20 mg/day of prednisone or equivalent). Moderate-risk level included patients without a level 1 condition but who had a history of chronic lung disease (chronic bronchitis, emphysema or asthma), liver disease (cirrhosis or alcoholic hepatitis), heart disease (congestive heart failure or chronic angina) and diabetes mellitus. Low-risk level included patients without the above mentioned risk conditions.
Pneumonia severity index (PSI) was calculated according to criteria described in classical meta-analysis, and case-fatality was considered when the patient died (in-hospital or not) within the first 30 days after the diagnosis of pneumonia[12, 13].
Penicillin-resistance was determined for early cases (2002–2004) and contemporary cases (2005–2007). For the statistical analysis, continuous variables were compared by Student's t test, whereas categorical values were compared using chi-squared or Fisher's test as appropriate.
The study was approved by the ethical committee of the Catalan Health Institute and was conducted in accordance with the general principles for observational studies set out by this institution (Exp FIS 050231).
Median age of case patients was 71.9 years (SD: 11.6), and 75 (72.1%) were male. Fifty-nine (56.7%) were 50–74 years-old, and 45 (43.3%) were 75 years or older. Five cases were managed as outpatient and 99 were hospitalised (20 of which required admission to the ICU). Overall case-fatality rate was 15.4% (40% among those admitted in the ICU).
Clinical characteristics of patients with pneumococcal pneumonia according to penicillin susceptibility.
N = 77
N = 27
N = 104
Age (yrs), mean (SD)
Chronic pulmonary disease
Chronic hearth disease
Chronic liver disease
Chronic renal disease
Risk strata of patients
S. Pneumoniae serotypes
Pneumococcal serotype was identified in 60 cases, whereas 14 cases were due to nonserotyped organisms and 30 were not serotyped. Of the 60 serotyped cases, 12 (20%) were due to type 3, seven (11.7%) due to type 1, four (6.7%) due to type 14, four (6.7%) due to type 6A, four (6.7%) due to type 8, three (5%) due to type 6B, three (5%) due to type 12F, two (3.3%) due to type 5, two (3.3%) due to serotype 4, two (3.3%) due to type 19F, two (3.3%) due to type 23A, two (3.3%) due to type 35B, two (3.3%) due to type 7F, two (3.3%) due to type 19A, two (3.3%) due to type 23B and one due to types 9V, 9N, 15B, 16, 23F, 33 and 38.
Of the total 104 cases, 27 (25.9%) were penicillin-resistant strains: 19 strains with intermediate resistance and 8 strains with high resistance (four cases with MIC = 2 μg/mL; four cases with MIC = 4 μg/mL)
Antimicrobial resistance to cefotaxime, erythromycin and levofloxacin observed among pneumococcal strains isolated over the study period.
All isolates (N = 104)
Penicillin resistant isolates (N = 27)
Only four (14.8%) isolates were identified as multidrug resistant S. pneumoniae (MRSP). Of these, one case was highly resistant to penicillin, cefotaxime and levofloxacin and three cases were highly resistant to penicillin, erythromycin and cefotaxime. Median age of patients with MRSP was 76.8 years, all of them had an underlying disease (one was current smoker, one had chronic heart disease and two had a chronic pulmonary disease), three were non-bacteremic cases and only one died. In three cases the microorganism was resistant to the initial antimicrobial regimen, and one of them died. Only two MRSP were serotyped (6B both of them).
Globally, 76 specimens were recovered from blood samples and 28 from sputum samples. Of the 42 resistant strains at least to one antibiotic, 26 (62%) were isolated from blood and 16 (38.1%) were from sputum. Although the difference did not reach statistical significance, penicillin resistance was substantially higher among Streptococcus pneumoniae recovered from sputum than from blood (44.4% vs. 24.2%, p = 0.186).
Comparing penicillin-susceptible and non-susceptible strains, statistically significant differences were observed only for age (table 1). Of the 19 serotyped strains with penicillin resistance, four (21.1%) were serotype 14, three (15.8%) were serotype 23B, three (15.8%) were serotype 6B, two (10.5%) were serotype 19F, two (10.5%) were serotype 6A, and one were serotypes 1, 3, 8, 9V and 35B.
Comparison of patients with pneumococcal pneumonia caused by penicillin-resistant strains in early and contemporary isolates according to their age and risk stratum.
75 yrs or more
Impact of resistance on severity outcomes
Mean length of hospital stay was slightly higher among patients infected with penicillin-resistant strains than in penicillin-susceptible strains (16.9 vs. 13.5 days; p = 0.358). PSI score did not differ in both groups (112.5 vs. 111.1; p = 0.838).
Overall mortality was 15.4%. One patient died among the 8 cases with an inadequate initial antimicrobial therapy, based on antibiogram results. Although it was not statistically significant, mortality was considerably higher among those patients who had an infection caused by a penicillin susceptible strain than in those with infections due to penicillin-resistant strains (16.9% vs. 11.1%; p = 0.474).
The antimicrobial therapies initially used in the 104 patients were: levofloxacin in 26 cases (25%), an association of macrolide and cephalosporin in 18 cases (17.3%), amoxicillin clavulanic acid in 14 cases (13.5%), a cephalosporin in 14 cases (13.5%), an association of macrolide and amoxicillin clavulanic acid in 14 cases (13.5%) and other treatments or associations in 18 cases (17.3%).
Considering the 8 cases classified as high-resistance to penicillin, antimicrobial therapy initially used after CAP diagnosis was levofloxacin in 3 cases, amoxicillin clavulanic acid in two cases, an association of macrolide and cephalosporin in two cases, and one case was treated with tazobactam. One patient (a man 88 years-old, PSI class V, treated with amoxicillin clavulanic acid, with bacteremic pneumococcal pneumonia due to serotype 6B, resistant to penicillin (MIC = 2) and resistant to cefotaxime, erythromycin and clindamycin) died two days after hospital admission, whereas the remaining 7 patients were non-fatality cases.
Considering the 19 cases classified as intermediate-resistance to penicillin, antimicrobial therapy initially used after CAP diagnosis was cephalosporin in 5 cases, amoxicillin clavulanic acid in 4 cases, an association of macrolide and cephalosporin in 4 cases, levofloxacin in 2 cases, and other treatments or associations in 4 cases. Two of these patients, both treated with a third generation cephalosporin, died within 10 days after hospital admission (one case was a man 67 years old with bacteremic pneumonia, PSI class V, due to serotype 14; and an other case was a man 76 years-old with bacteremic pneumonia, PSI class IV, due to serotype 6A), whereas the remaining 17 penicillin intermediate resistance cases were non-fatality cases.
In the present study, the overall rate of penicillin-resistance among isolates of Streptococcus pneumoniae was considerable (25.9%). The proportion of penicillin-resistance was higher among early isolates than in contemporary isolates, which concurs with other Spanish studies[5, 7, 14]. Among Spanish individuals, Fenoll et al found that a high-level penicillin-resistance rate (MIC > 2 mg/l) decreased from 13.3% in 1997 to 12.9% in 2001. In Catalonia, the high-level penicillin-resistance fell from 15% (1989–1993) to 8.9% (1999–2002), which fits with the 9% found in our study. In contrast, the results found in other countries showed an increase in penicillin-resistance rate. In United States, the high-level penicillin-resistance increased from 14.7% (1998–1999) to 18.4% (2001–2002). Although several factors explain the emergence of drug resistant Streptococcus pneumoniae, the single most important consideration, however, is the selective pressure of use of antimicrobial agents. Reductions in the rate of resistance found in our study could be explain by a reduction in consumption of antibiotics in Catalonia, going from 17.77 defined daily doses per 1000 inhabitants per day (DHD) in 1999 to 12.58 DHD in 2006, and could be partially attributed to the vaccination that covers serotypes typically associated with drug resistance.
We found a statistically higher age among patients infected with penicillin-resistant strains than in those infected with penicillin-susceptible strains. However, because of low statistical power, we could not reject that prevalence of underlying conditions was the predisposing cause for this age-related differences in the aetiology of pneumococcal pneumonia.
In accordance with previous studies[5, 14] we found a high proportion of underlying diseases among patients with pneumococcal pneumonia. Although not statistically significant, the proportion of penicillin resistance in our study was higher among patients with low or moderate level risk in early isolates. Conversely, in contemporary isolates this proportion was higher among patients in high level of risk. Some studies[6, 16] have reported that patients with chronic pulmonary disease, HIV infected, suspected aspiration or who were admitted to the hospital within the previous 3 months were more likely than other patients to have penicillin resistant pneumococcal pneumonia. In our study, only two patients had been hospitalized in prior 3 months, and none of them were HIV infected. Chronic pulmonary disease was highly prevalent in our study but differences were not observed in prevalence by penicillin resistance status.
Almost three quarter (74.1%) of penicillin-resistant pneumococcal pneumonia were PSI classes IV-V. There was a similar percentage of penicillin-resistant pneumonia between PSI classes IV-V and PSI classes I-III. This results agree with those found in other studies[6, 16] that only reported a trend for higher PSI classes to be associated with penicillin-resistant pneumococcal pneumonia.
In the present study, the rate of penicillin susceptibility was higher in bacteremic than non-bacteremic cases (65.8% vs. 42.9%). These fits with Aspa's report, where penicillin susceptibility was 71% among blood culture isolates and 50% among sputum isolates. In the USA, a higher prevalence of resistance to penicillin among lower respiratory tract than among isolates recovered from blood samples (16.2% vs. 13.7%) has also been reported.
In our study, most penicillin resistant S. pneumoniae isolates were resistant to at least one additional antimicrobial agent, and 3.8% (four cases) of all isolates were identified as MRSP. As described in previous studies penicillin resistance, particularly high-level resistance, predicts resistance to other antimicrobials[11, 14, 17–19]. We found that all MRSP were highly resistant to penicillin and cefotaxime, and three of them were highly resistant to erythromycin too.
We found that an inadequate initial antimicrobial treatment was started in 7.7% of patients. Although in our study there was no excess mortality due to initial inappropriate treatment (12.5%), likely due to a small sample size, it is important to take into account underlying condition or regional pattern of drug resistance for S. pneumoniae in determining adequate antimicrobial therapy[11, 17, 20].
In accordance with other studies[5, 14, 21, 22], penicillin resistance was not related with higher mortality. It has been suggested that the primary determinant of virulence might be the serotype rather than its sensitivity to penicillin, and it is possible that the mechanisms conferring penicillin resistance are related to those leading to a loss of virulence. Previous studies of pneumococcal bacteremia did not show differences in mortality between those with susceptible and those with non-susceptible pneumococci after adjustment for age, underlying disease, severity of illness on presentation, and appropriate concordant treatment[5, 6, 16].
We found that three serotypes (14, 6B and 23B) were associated with increasing risk of penicillin resistance, accounting for a 53% of serotyped pneumococci causing penicillin-resistant infections and the serotype 6B was responsible for 50% of cases of MRSP. In accordance with our results, Fenoll et al reported that 56% of penicillin-resistant pneumococcal strains belonged to serotypes 6 (18%), 14 (19%) and 19 (19%). In a long term follow-up between 1983 and 2003 Mufson et al found that all blood isolates of penicillin-resistant were capsular serotypes 6, 9, 14, 19 and 23.
Main limitation in this study includes the relatively low number of cases and the difficulty to generalize results. According to classical breakpoint criteria, it could be concluded that, in the study area, resistance to penicillin among Streptococcus pneumoniae remains high, although such resistance does not result in increased severity or mortality. However, it must be emphasized that, according to the new breakpoint criteria for intravenous penicillin, almost all strains in our study would be susceptible.
To date, criteria for penicillin susceptibility of Streptococcus pneumoniae remains unchanged for patients without meningitis who can be treated with oral penicillin (e.g., for outpatient pneumonia). However, since 2008, for patients without meningitis who are treated with intravenous penicillin, the new breakpoints are ≤ 2, 4, and ≥ 8 μg/mL for susceptibility, intermediate-resistance and high-resistance, respectively. Thus, considering the new pneumonia breakpoints, 100 (96.2%) of the 104 cases in our study would be penicillin-susceptible (MIC ≤ 2 μg/mL), 4 (3.8%) would be penicillin intermediate-resistant (MIC = 4 μg/mL) and none would be high resistance (MIC ≥ 8 μg/mL).
According to the change in pneumonia breakpoint for susceptibility, percentages of strains resistant to penicillin have dramatically decreased worlwide. In Spain, a large surveillance study that included 2,721 isolates of S. Pneumoniae obtained from November 2001 to October 2002 in diferent Spanish Hospitals reported that (taking into account the old pneumonia breakpoint) 20% of overall strains were penicillin resistant (MIC >2 mg/mL) and 24% were intermediate resistant strains (MIC = 0.12–1 mg/mL). However, if we consider the new pneumonia breakpoint, 94.3% of the total strains in the forementioned study would be currently considered penicillin susceptible, which is also in agreement with data found in the present study (96.2% penicillin susceptible strains in our study considering the new breakpoint).
According to classical pneumonia breakpoints for susceptibility to penicillin, the resistance among Streptococcus pneumoniae was considerable, but with the use of new breakpoints, the percentage of Streptococcus pneumoniae penicillin-resistant decreases dramatically.
In vitro penicillin resistance is not associated with increasing mortality, even considering new breakpoints for susceptibility to intravenous penicillin.
The low mortality rates reported among patients with pneumonia due to penicillin resistant strains could likely be due to the fact that patients were treated with active agents, such as penicillins or third generation cephalosporins.
This study was supported by a grant from the "Fondo de Investigación Sanitaria" of the Spanish Health Ministry (expedient FIS-050231).
- Aspa J, Rajas O, de Castro FR: Pneumococcal antimicrobial resistance: therapeutic strategy and management in community-acquired pneumonia. Expert Opin Pharmacother. 2008, 9 (2): 229-41. 10.1517/146565126.96.36.199.View ArticlePubMedGoogle Scholar
- Metlay JP: Antibacterial drug resistance: implications for the treatment of patients with community-acquired pneumonia. Infect Dis Clin North Am. 2004, 18 (4): 777-90. 10.1016/j.idc.2004.07.003.View ArticlePubMedGoogle Scholar
- García-Rey C, Fenoll A, Aguilar L, Casal J: Effect of social and climatological factors on antimicrobial use and Streptococcus pneumoniae resistance in different provinces in Spain. J Antimicrob Chemother. 2004, 54: 465-71. 10.1093/jac/dkh375.View ArticlePubMedGoogle Scholar
- Tleyjeh IM, Tlaygeh HM, Hejal R, Montori VM, Baddour LM: The impact of penicillin resistance on short-term mortality in hospitalized adults with pneumococcal pneumonia: a systematic review and meta-analysis. Clin Infect Dis. 2006, 42: 788-97. 10.1086/500140.View ArticlePubMedGoogle Scholar
- Pallares R, Liñares J, Vadillo M, Cabellos C, Manresa F, Viladrich PF, Martin R, Gudiol F: Resistance to penicillin and cephalosporin and mortality from severe pneumococcal pneumonia in Barcelona, Spain. N Engl J Med. 1995, 333: 474-80. 10.1056/NEJM199508243330802.View ArticlePubMedGoogle Scholar
- Aspa J, Rajas O, Rodríguez de Castro F, Blanquer J, Zalacain R, Fenoll A, de Celis R, Vargas A, Rodríguez Salvanés F, España PP, Rello J, Torres A, Pneumococcal Pneumonia in Spain Study Group: Drug-resistant pneumococcal pneumonia: clinical relevance and related factors. Clin Infect Dis. 2004, 38: 787-98. 10.1086/381886.View ArticlePubMedGoogle Scholar
- Fenoll A, Asensio G, Jado A, Berrón S, Camacho MT, Ortega M, Casal J: Antimicrobial susceptibility and pneumococcal serotypes. J Antimicrob Chemother. 2002, 50 (suppl S2): 13-19. 10.1093/jac/dkf509.View ArticlePubMedGoogle Scholar
- Garau J: Treatment of drug-resistant pneumococcal pneumonia. Lancet Infect Dis. 2002, 2 (7): 404-15. 10.1016/S1473-3099(02)00316-X.View ArticlePubMedGoogle Scholar
- Neuman MI, Kelley M, Harper MB, File TM, Camargo CA, EMNet Investigators: Factors associated with antimicrobial resistance and mortality in pneumococcal bacteremia. J Emerg Med. 2007, 32 (4): 349-57. 10.1016/j.jemermed.2006.08.014.View ArticlePubMedPubMed CentralGoogle Scholar
- Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement M100-S17. 2007, 27: 126-8.Google Scholar
- Clavo-Sánchez AJ, Girón-González JA, López-Prieto D, Canueto-Quintero J, Sánchez-Porto A, Vergara-Campos A, Marin-Casanova P, Córdoba-Doña JA: Multivariate analysis of risk factors for infection due to penicillin-resistant and multidrug-resistant Streptococcus pneumoniae: a multicenter study. Clin Infect Dis. 1997, 24 (6): 1052-9. 10.1086/513628.View ArticlePubMedGoogle Scholar
- Fine MJ, Auble TE, Yealy DM, Hanusa BH, Weissfeld LA, Singer DE, Coley CM, Marrie TJ, Kapoor WN: A prediction rule to identify low-risk patients with community-acquired pneumonia. N Engl J Med. 1997, 336: 243-50. 10.1056/NEJM199701233360402.View ArticlePubMedGoogle Scholar
- Fine MJ, Smith MA, Carson CA, Meffe F, Sankey SS, Weissfeld LA, Detsky AS, Kapoor WN: Efficacy of pneumococcal vaccination in adults. A meta-analysis of randomized controlled trials. Arch Intern Med. 1994, 154: 2666-77.View ArticlePubMedGoogle Scholar
- Vallès X, Marcos A, Pinart M, Piñer R, Marco F, Mensa JM, Torres A: Hospitalized Community-Acquired Pneumonia due to Streptococcus pneumoniae. Has resistance to antibiotics decreased?. Chest. 2006, 130: 800-806. 10.1378/chest.130.3.800.View ArticlePubMedGoogle Scholar
- Karlowsky JA, Thornsberry C, Jones ME, Evangelista AT, Critchley IA, Sahm DF: Factors associated with relative rates of antimicrobial resistance among Streptococcus pneumoniae in United States: Results from the TRUST Surveillance Program (1998–2002). Clin Infect Dis. 2003, 36: 963-70. 10.1086/374052.View ArticlePubMedGoogle Scholar
- Falcó V, Almirante B, Jordano Q, Calonge L, del Valle O, Pigrau C, Planes AM, Gavaldà J, Pahissa A: Influence of penicillin resistance on outcome in adults patients with invasive pneumococcal pneumonia: is penicillin useful against intermediately resistant strains?. J Antimicrob Chemother. 2004, 54: 481-8. 10.1093/jac/dkh338.View ArticlePubMedGoogle Scholar
- Neralla S, Meyer KC: Drug treatment of pneumococcal pneumonia in the elderly. Drugs Aging. 2004, 21 (13): 851-64. 10.2165/00002512-200421130-00003.View ArticlePubMedGoogle Scholar
- Fenoll A, Giménez MJ, Robledo O, Aguilar L, Tarragó D, Granizo JJ, Martín-Herrero JE: Influence of penicillin/amoxicillin non-susceptibility on the activity of third-generation cephalosporins against Streptococcus pneumoniae. Eur J Clin Microbiol Infect Dis. 2008, 27 (1): 75-80. 10.1007/s10096-007-0402-5.View ArticlePubMedGoogle Scholar
- Riedel S, Beekmann SE, Heilmann KP, Richter SS, Garcia-de-Lomas J, Ferech M, Goosens H, Doern GV: Antimicrobial use in Europe and antimicrobial resistance in Streptococcus pneumoniae. Eur J Clin Microbiol Infect Dis. 2007, 26 (7): 485-90. 10.1007/s10096-007-0321-5.View ArticlePubMedGoogle Scholar
- Campbell GD, Silberman R: Drug-resistant Streptococcus pneumoniae. Clin Infect Dis. 1998, 6 (5): 1188-95. 10.1086/520286.View ArticleGoogle Scholar
- Yu VL, Baddour LM: Infection by drug-resistant Streptococcus pneumoniae is not linked to increased mortality. Clin Infect Dis. 2004, 39 (7): 1086-7. 10.1086/423812.View ArticlePubMedGoogle Scholar
- Song JH, Oh WS, Kang CI, Chung DR, Peck KR, Ko KS, Yeom JS, Kim CK, Kim SW, Chang HH, Kim YS, Jung SI, Tong Z, Wang Q, Huang SG, Liu JW, Lalitha MK, Tan BH, Van PH, Carlos CC, So T, Asian Network for Surveillance of Resistant Pathogens Study Group: Epidemiology and clinical outcomes of community-acquired pneumonia in adult patients in Asian countries: a prospective study by the Asian network for surveillance of resistant pathogens. Int J Antimicrob Agents. 2008, 31 (2): 107-14. 10.1016/j.ijantimicag.2007.09.014.View ArticlePubMedGoogle Scholar
- Mufson MA, Chan G, Stanek RJ: Penicillin resistance not a factor in outcome from invasive Streptococcus pneumoniae community-acquired pneumonia in adults when appropriate empiric therapy is started. Am J Med Sci. 2007, 333 (3): 161-7. 10.1097/MAJ.0b013e3180312cd5.View ArticlePubMedGoogle Scholar
- Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing; eighteenth informational supplement. CLSI document M100-S18. 2008, Wayne, PA: Clinical and Laboratory Standards InstituteGoogle Scholar
- Pérez-Trallero E, García de la Fuente C, García-Rey C, Baquero F, Aguilar L, Dal-Re R, García-de-Lomas J, Spanish Surveillance Group for Respiratory Pathogens: Geographical and ecological analysis of resistance, coresistance, and coupled resistance to antimicrobials in respiratory pathogenic bacteria in Spain. Antimicrob Agents Chemother. 2005, 49: 1965-72. 10.1128/AAC.49.5.1965-1972.2005.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/9/36/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.