From cat scratch disease to endocarditis, the possible natural history of Bartonella henselaeinfection
© Gouriet et al; licensee BioMed Central Ltd. 2007
Received: 25 September 2006
Accepted: 18 April 2007
Published: 18 April 2007
Most patients with infectious endocarditis (IE) due to Bartonella henselae have a history of exposure to cats and pre-existing heart valve lesions. To date, none of the reported patients have had a history of typical cat scratch disease (CSD) which is also a manifestation of infection with B. henselae.
Here we report the case of a patient who had CSD and six months later developed IE of the mitral valve caused by B. henselae.
Based on this unique case, we speculate that CSD represents the primary-infection of B. henselae and that IE follows in patients with heart valve lesions.
Bartonella spp. are link to the host immune system, infection with the same Bartonella species (e.g., B. henselae) can result in focal suppurative reaction (cat scratch disease in immunocompetent patients), a multifocal angioproliferative response (bacillary angiomatosis in immunocompromised patients), an increased immune response without evidence of bacteria in patient tissues (meningoencephalitis), or endovascular multiplication of the bacteria (endocarditis). Of the 19 species within the genus Bartonella , seven are known to cause infective endocarditis (IE) in people: B. quintana , B. henselae , B. elizabethae , B. vinsonii subps. berkhoffii , B. vinsonii subps. arupensis , B. kohlerae  and B. alsatica . These zoonotic agents cause 1 to 15% of all cases of IE [3, 9, 10] and cannot be detected using routine blood cultures due to the fastidious nature of the bacteria. The most widely used method for the laboratory diagnosis of infection with Bartonella sp. is serology. Enzyme immunoassay (EIA) has been described  but indirect immunofluorescence antibody assay (IFA) is the reference technique , despite cross-reactivity among Bartonella spp. . Our laboratory uses two IFA titers in the diagnosis of Bartonella infections. In conjunction with a compatible medical history, an IgG titer ≥1:50 to B. henselae suggests a diagnosis of acute infections such as cat scratch disease (CSD), while IgG ≥1:800 to either B. henselae or B. quintana suggests a diagnosis of endocarditis [14, 15]. Western-blotting with adsorbed sera enables us to differentiate between infections with B. henselae and B. quintana . Currently, the literature describes B. henselae as an agent causing a typical endocarditis which is easily diagnosed using the Duke criteria [3, 16] and usually with vegetations that can be detected by echocardiography. Patients usually have a pre-existing cardiac valve lesion and although they are exposed to cats, they usually do not have a history of CSD. Here we report a patient who suffered from CSD and subsequently developed B. henselae IE.
The diagnosis of IE was made retrospectively based on the combination of histology of the cardiac valve lesions, culture of Bartonella from the valve, presence of a predisposing heart condition, and serological evidence of Bartonella infection. Without the histology of the valve the patient would not have had a positive score using the Duke criteria; he would only have had 2 minor criteria. After surgery, the patient recovered rapidly with routine post-surgical amoxicillin administration for 4 days, followed by gentamycin for 15 days and doxycycline for 1 month .
Retrospectively, it was found that six months before the patient had had suspected lymphoma of an inguinal lymph node. Histology of the node, however, showed a necrotizing lymphadenitis suggested of CSD. Numerous microabscesses containing fragmented neutrophils were observed in homogenous necrotic areas. These necrotic regions were surrounded by a ring of macrophages and epithelioid histiocytes to form stellate inflammatory granulomas (Figure 1). No bacteria were detected by immunohistochemical examination or Warthin-Starry staining. Unfortunately, the lymph node sample was not available for PCR analysis to confirm the diagnosis of CSD.
The patient did not own a cat but reported a single contact with a stray cat that scratched him one month before the enlargement of the inguinal lymph node. We report the development of IE after a likely episode of CSD in a patient with a mechanical mitral cardiac valve. In previous studies , B. henselae was described in patients who have regular contact with cats and with pre-existing valvulopathies [24, 25], but to the best of our knowledge the progression of CSD to IE has not previously been reported.
We describe here the development of IE six months after CSD in a patient with a known valvulopathy. The diagnosis of CSD was histological as blood collected at the time was not available for serological or DNA testing. Serology in May 2005, however, revealed a titer of 1:200 against the Bartonella antigens, which is intermediate between the titer we use to diagnose CSD and that for IE . Most patients with Bartonella IE have high titers >800 . However, in our case the western-blotting showed an endocarditis profile (Figure 2) and cross adsorption confirmed the diagnosis of B. henselae infection . Also, B. henselae genotype five was identified by both PCR and culture of the cardiac valve. The possibility of chronic bacteremia due to B. henselae has been reported in a patient during the natural course of CSD . The isolation of the Bartonella species is difficult, especially in immunocompetent patients, and cultures of clinical samples are most often sterile. The best specimens for PCR amplification are cardiac valve biopsies, but these are seldom available. Testing can be performed even following antibiotic therapy. Six months after our patient likely suffered from CSD, we diagnosed asymptomatic IE with degenerative lesions in the valve but no vegetations were found echocardiographically.
Therefore, the natural history of B. henselae may resemble that of Q fever . As with B. henselae, the majority of patients with Q fever IE have pre-existing valve lesions . Between 30 and 50% of patients with cardiac valve lesions that have primary infections with Coxiella burnetii (symptomatic or asymptomatic) develop IE within 2 years . However, the delay between bacteremia and IE ranges from months to years and we now recommend that the presence of valve lesions be carefully investigated in patients with Q fever as this enables early treatment .
This single case report provides further insight into the natural history of B. henselae IE. To our knowledge this is the first report of the progression of probable CSD to B. henselae IE in a patient with a pre-existing cardiac valve lesion and only a single contact with a cat. It shows that contact with a cat and CSD are risk factors for IE in patients with cardiac valve disease. Our findings suggest echocardiography may be indicated in patients with CSD. Follow-up of patients with cardiac valve lesions that develop CSD may enable the early treatment of B. henselae IE.
We thank Mireille Papadacci and Jean-Paul Casalta for help in collecting data. We thank Pat Kelly for reviewing the manuscript. Written consent was obtained from the patient for publication of the study. We thank Wenjun Li and Pierre-Edouard Fournier fo the B. henselae sequencing.
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